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conferenceseries
.com
June 26-27, 2017 San Diego, USA
13
th
International conference on
Pathology and Molecular Diagnosis
Volume 7, Issue 2 (Suppl)
J Clin Exp Pathol, an open access journal
ISSN:2161-0681
Pathology and Molecular Diagnosis 2017
June 26-27, 2017
A Duo-toehold-mediated displacement amplification on DNA tetrahedron for RNA detection of
dengue virus
Sheng Xi Chen, Ming Xuan Gao
and
Sidney M. Hecht
Arizona State University, USA
D
engue is the most rapidly spreading endemic viral disease in the subtropical and tropical area of the world.This disease is
threatening more than 2.5 billion people in more than 100 countries. Here, a novel duo-toehold-mediated displacement
amplification strategy on DNA tetrahedron has been developed for sensitively detecting RNA of dengue virus. In this strategy,
protector DNAwas annealed to the tetrahedron to form a FRET-ON status at initial stage. When targets presented, the protector
could be displaced by the target and switch to FRET-OFF. Meanwhile, the targets could be displaced again by the capture DNA
and recycled in the process to amplify the signal. Simply by using the fluorescence spectrometer, the detection limit could be as
low as 10 pM, which was more sensitive by 3 orders of magnitude than traditional non-amplified detecting methods.
Biography
Shengxi Chen has completed his PhD at the age of 27 years from Peking Union Medical Collegeand postdoctoral studies from University of Virginia. He is the
Associate Professor Research in Center for BioEnergetics, Biodesign Institute, Arizona State University. He has published more than 30 papers in reputed journals.
shengxi.chen.1@asu.eduSheng Xi Chen et al., J Clin Exp Pathol 2017, 7:2 (Suppl)
DOI: 10.4172/2161-0681-C1-034