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conferenceseries
.com
Volume 8
Journal of Obesity & Weight Loss Therapy
Obesity Meeting 2018
August 24-25, 2018
August 24-25, 2018 Singapore
20
th
Global Obesity Meeting
Ping Zhou et al., J Obes Weight Loss Ther 2018, Volume 8
DOI: 10.4172/2165-7904-C6-072
Notoginsenoside R1 attenuates diabetic retinopathy
via
TFEB-dependent enhancement of mitophagy flux
Ping Zhou, Weijie Xie, Gui-bo Sun and Xiao-bo Sun
Peking Union Medical College, China
A
growing evidence have proved that dysfunction of mitophagy in retinal micro-vascular endothelial cells is associated with
Diabetic Retinopathy (DR). Notoginsenoside R1 (NGR1) is isolated from
P. notoginseng
and has many pharmacological
effects such as anti-inflammatory, anti-oxidative and anti-apoptotic properties. However, its protective effects on DR and the
underlying mechanism are still unknown. In the present study, we found that NGR1 could significantly attenuate DR in db/
db mice, characterized by the reduced micro-aneurysm in the retina and increased amplitudes of B-wave. NGR1 pretreatment
also significantly inhibited apoptosis in RF/6A cell model of hyperglycemia, which were detected by TUNEL and Annexin
V/PI staining. NGR1 markedly reduced the production of VEGF, remarkably augmented the level of PEDF and significantly
suppressed oxidative stress and inflammation in RF/6A cells exposed to HG and the retinas of db/db mice. Moreover, the
increased PINK1 and Parkin expression, the elevated LC3-II/LC3-I ratio, and the lessened p62/SQTSM1 expression were
observed in NGR1-treated RF/6A cells exposed to HG and the retinas of NGR1-treated db/db mice. Furthermore, NGR1
pretreatment promoted TFEB nuclear translocation, which resulted in up-regulation of LAMP-1 in RF/6A cells and the retinas
of db/db mice. NGR1 pretreatment also increased co-localization of LAMP-1 and Tomm20 in RF/6A cells. Importantly, the
knockdown of TFEB could abolish these protective effects of NGR1. In summary, these results demonstrated that NGR1
protected against DR
via
TFEB-dependent enhancement of mitophagy flux.
Biography
Ping Zhou is currently pursuing her PhD from the Institute of Medicinal Plant Development, Peking Union Medical College, Beijing, China. She has majored in
Pharmacology of Traditional Chinese Medicine and her main focus is on the study of pathogenesis of diabetes and its complications and the protective effect of
Panax notoginseng.
zhoup0520@163.com