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Volume 8 Journal of Obesity & Weight Loss Therapy Obesity Meeting 2018
August 24-25, 2018
August 24-25, 2018 Singapore
20th Global Obesity Meeting
Leptin administration in mid-pregnancy reduces fetal and early postnatal growth rate and abolishes obesity-
associated hyperglycemia in mouse offspring
Elena Makarova, Denisova E and Kozhevnikova V
Institute of Cytology and Genetics, Russia
Maternal obesity increases the risk of obesity in offspring. Leptin is increased in obese animals, and elevation of maternal
leptin may affect the metabolic phenotype of the offspring. We explore the effects of leptin elevation during mid-
pregnancy on the offspring metabolic phenotypes, fetal growth, and placental gene expression. C57BL mice received a single
injection of leptin or saline on pregnancy day 12. Body Weight (BW) was measured weekly in offspring, which consumed
standard show or palatable food and the mRNA expression of glucose and amino acid transporters, insulin-like growth factor
2 and its receptor was measured 3 hours and the placental and fetal weights were measured 24 hours after the injection. The
offspring born to leptin-treated mothers exhibited growth retardation before and catch-up growth after weaning and mature
male offspring had an increased BW on a standard diet. Prenatal exposure to leptin did not influence the obesity development
but prevented the development of obesity-associated hyperglycemia. The leptin injection decreased the fetal weight by 5%
and the placental mRNA level of amino acid transporter SNAT2. The results suggest that elevation of maternal leptin in mid-
pregnancy has positive effect on glucose metabolism in mature offspring and this effect is associated with leptin influence on
fetal growth and amino acid transporter expression in placentas. Supported by the RFBR, Grant 17-04-01357.
Biography
Elena Makarova is currently a Senior Researcher in the Laboratory of Physiological Genetics in the Institute of Cytology and Genetics, Novosibirsk, Russia. Her
researches focus on the studies of sex-specific influence of maternal leptin on metabolic characteristics in progeny of rodents. She with her colleagues found
maternal leptin retarded obesity development in male progeny and improves glucose metabolism in progeny of both sexes in mice.
Elena Makarova et al, J Obes Weight Loss Ther 2018, Volume 8
DOI: 10.4172/2165-7904-C6-073