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Journal of Infectious Diseases & Therapy | ISSN: 2332-0877 | Volume 6
&
Nosocomial and Healthcare Associated Infections
2
nd
International Congress on
Decontamination, Sterilization and Infection Control
International Conference on
October 15-16, 2018 | Las Vegas, USA
Molecular detection of virulence factors in antibiotic resistant
enterococcus
faecalis
from abattoir,
poultry and clinic
Adetunji Kola Olawale
Afe Babalola University, Nigeria
E
nterococcus faecalis
is a major cause of nosocomial infection in human and has been linked to severe extra-intestinal
infections in animals. This study determines the frequency and distribution of antibiotic-resistant
Enterococcus faecalis
(ARE) in poultry, abattoir and clinical environment. A total of the 150 samples including; poultry droppings, abattoir waste-
water, and clinical specimens were collected. Standard bacteriological methods were used in isolation and characterization
of
E. faecalis
, while disc diffusion technique was used in determining antibiotic resistance pattern of the isolates. Of the 150
samples examined, 53% were positive for
E. faecalis
. The highest occurrence (31.33%) of
E. faecalis
was recorded from poultry
samples, followed by (14%) from abattoir samples and the least (8%) from clinical samples. The High antibiotic resistance
ranging between 33.3% and 100% were recorded.
E. faecalis
isolates from abattoir shown the highest percentage antibiotic
resistance, followed by clinical isolates and least among the poultry isolates. Cefuroxime, Erythromycin and Augmentin were
less effective against selected
E. faecalis
isolates while Ofloxacin was highly effective. Molecular detection of each of the genes
coding virulence factors - enterococcal surface protein (esp), aggregation substance (asa1) and collagen-binding protein (
ace
)
in E.faecalis revealed; presence in four isolates (EKSG-3, EKSG-7, EKSG-11, EKSG-20), three isolates (SP2B1, SP2A1, PKL-41)
and none from abattoir, poultry and clinical samples respectively. The study revealed high incidence of antibiotic resistant
E.
faecalis
with virulence potentials in the studied areas especially abattoir which could serve as a reservoir for antibiotic-resistant
strains. Hence, need for enforcement of good hygiene practice and constant epidemiological surveillance.
Biography
A K Olawale lectures as a Senior Lecturer in the Department of Biological Sciences (Microbiology and Biotechnology unit), Afe Babalola University, Ado-Ekiti.
Nigeria. He has PhD in Medical Microbiology. He possessed excellent experience in the clinical diagnosis and scientific research in molecular epidemiology and
pathogenesis, emerging infectious diseases, antimicrobial resistance, pathogens fingerprinting, molecular analysis of human pathogen virulence, development
of new antimicrobials and immunology. He has made some modest contributions in these major areas of Microbiology with notable publications in peer-reviewed
learned journals.
olawaleadetunji@abuad.edu.ngAdetunji Kola Olawale, J Infect Dis Ther 2018, Volume 6
DOI: 10.4172/2332-0877-C4-047