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Journal of Ecosystem & Ecography | ISSN: 2157-7625 | Volume: 8
June 28-29, 2018 | Alexandria, Egypt
International Pre Conference Workshop on
Microbial Ecology & Eco Systems
Isolation and identification of predominant bacterial isolates infecting urinary tract
1Marwa M. Elmaghrabi and 2Hanan A. Ghozlan
1Stem Cells and Tissue Culture Labs, CERRMA, Faculty of Medicine, Alexandria University, Alexandria, Egypt
2Faculty of Science, Alexandria University, Alexandria, Egypt
In this study, bacterial isolates of the most common urinary tract infection of 100 patients were investigated. Patients
comprised of equal gender and 50 patients were above 40 y/o and 50 were under 40 y/o. Only 55 patients were infected of
whom 63.6% females and 36.4% males, and among them 66% were above 40 y/o, and 44% were under 40 y/o. The dipstick test
revealed 24% were positive for leukocytes, 13% were positive for nitrite, 14% were positive for both leukocyte and nitrite. Phase
contrast microscopy revealed 15% were positive for pyuria, and 34% were positive for bacterurea. Morphological description
leucocytosis and bacterurea colonies grown on cultured on MacConkey and Blood agar plates was achieved, where 64 bacterial
strains and four fungal strains were identified. Based on Gram staining and cell shapes, isolates were grouped into three
categories; Gram (+) cocci, Gram (-) coccobacilli, and Gram (-) bacilli, and analyzed using SYSTAT® program. Following
cluster analysis, a representative strain of each cluster was selected for identification using VITEK® system. Results showed
eight groups of isolates; 28 E. coli, 9 Klebsiella pneumonia, 6 Pseudomonas aeruginosa, 6 Proteus mirabilis, 5 Staphylococcus
aurous, 4 Enterococcus faecalis, 4 Morganella morganii and 2 Pseudomonas fluorescence. Large cells of Candida albicans were
also identified. Results indicated that the most predominant uropathogen was E. coli as it was found in 43.7% of the isolates
followed by Klebsiella Pneumonia 14.1%. Both Pseudomonas aeruginosa and Proteus mirabilis were represented in 9.4% of the
isolates while Staphylococcus aurous was recorded in 7.8% of the samples. Enterococcus faecalis and Morganella morganii
were represented in 6.2% of the isolates however, only 3.2% was recorded for Pseudomonas fluorescence.
Keywords: bacterial description, SYSTAT®program, urinary tract infection, VITEK® system
Biography
Marwa M. Elmaghrabi is currently a permanent researcher at Stem Cells and Tissue Culture Labs, Faculty of Medicine, Alexandria University, a healthcare and
quality advisor at Canadian Academy of Sciences, Egypt. She has MSc in Microbiology (2012), Faculty of Science, Egypt. She accumulated 8-years of experience
in quality and infection control, and appointed to a number of key jobs; ISO 9001:2015 Lead Auditor, quality manager (2015-2017) and quality and infection control
manager (2013-2015) in Madina Fertility group, quality-specialist at Medical Research Institute, Alexandria University, 2015-2017, and senior quality assurance
specialist and internal auditor at Hassab-Labs Company, 2010-2013. She participated in a number of regional and international conferences and as a member
of Organizing Committee of Microbial Ecology-2018 pre-conference workshop. She contributed to PAN-African and electronic network project as a broadcasting
lecturer. She served as a member of the Egyptian Syndicate of Scientific professions, and Arab QOSH of safety professionals' experts.
Marwa M. Elmaghrabi et al., J Ecosys Ecograph 2018, Volume: 8
DOI: 10.4172/2157-7625-C2-035