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Volume 3
Diagnostic Pathology: Open Access
ISSN: 2476-2024
Laboratory Medicine 2018
June 25-26, 2018
Page 37
Notes:
conference
series
.com
June 25-26, 2018 | Berlin, Germany
13
th
International Conference on
Laboratory Medicine & Pathology
Claudio Sorio, Diagn Pathol Open 2018, Volume 3
DOI: 10.4172/2476-2024-C1-001
Role of of protein tyrosine phosphatase receptor type γ in chronic myeloid leukemia
P
rotein tyrosine phosphatase receptor gamma (PTPRG) is a ubiquitously expressed member of the protein tyrosine phosphatase
family known to act as a tumor suppressor gene in many different neoplasms with mechanisms of inactivation including
mutations and methylation of CpG islands in the promoter region. We identified a critical role in human hematopoiesis and
describe a role as oncosuppressor in chronic myeloid leukemia (CML). We have described PTPRG expression in various tissues
and recently developed a monoclonal antibody capable of recognizing the native antigen of this phosphatase by flow cytometry: we
confirmed PTPRG protein downregulation in CML patients at diagnosis in the Philadelphia-positive myeloid lineage (including
CD34+/CD38bright/dim cells). After effective tyrosine kinase inhibitor (TKI) treatment, its expression recovered in tandem with
the return of Philadelphia-negative hematopoiesis. Of note, PTPRG mRNA levels remain unchanged in tyrosine kinase inhibitors
(TKI) non-responder patients, confirming that downregulation selectively occurs in primary CML cells. We have also identified a
novel regulative loop involving CTNNB1 gene. The availability of this unique antibody permits its evaluation for clinical application
including the support for diagnosis and follow-up of these disorders. Evaluation of the role of PTPRG in health and disease is
facilitated by the availability of a specific reagent capable to specifically detect its target in various experimental conditions.
Biography
Claudio Sorio has completed his MD from University of Verona, Italy and his PhD and Postdoctoral studies from Thomas Jefferson University, Philadelphia, USA. He
specilized in Surgical Pathology at the same university. He is the Director of Cystic Fibrosis Traslational Research Laboratory “D Lissandrini” at the University of Verona
and Head of the Biomarker Laboratory at the same institution. He has published more than 74 original papers and several reviews and book chapters in reputed journals
and has been serving as an Editorial Board Member of reputed journals.
claudio.sorio@univr.itClaudio Sorio
University of Verona, Italy