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Volume 6 Journal of Infectious Diseases & Therapy-Open Access Infectious Diseases Congress 2018
November 15-16, 2018
November 15-16, 2018 Istanbul, Turkey
6th Annual Conference on
Parasitology & Infectious Diseases
Mesenchymal stem cell as a cure for brain damage induced by chronic Toxocara canis infection in an
experimental mouse model
Fatma Hamed Shalan1, Ayman Abd El-Moenem Elbadry2, Amera Fathy Afifi 1, Engy Victor Nassief Beshay 1, Samar Ahmed El-Refai1, Hala Gabr Metwaly3,
Eman Ahmedy4, Eman Abd El-Fattah Badr 5, Dalia Refaat Al-Sharaky6 and Gehan Salah Sadek1
1Parasitology Department, Faculty of Medicine, Menoufia University, Egypt
2Parasitology Department, Faculty of Medicine, Cairo University, Egypt
3Clinical Pathology Department, Faculty of Medicine, Cairo University, Egypt
4Clinical Pathology Department, Faculty of Medicine, Menoufia University, Egypt
5Biochemistry Department, Faculty of Medicine, Menoufia University, Egypt
6Pathology Department, Faculty of Medicine, Menoufia University, Egypt
Neurotoxocariasis is a serious condition linked to the reduced cognitive function in children and some neurodegenerative
diseases. Unfortunately, the available drugs for its treatment are with variable results. Mesenchymal stem cells (MSCs)
have been used in experimental and clinical trials and it gave promising therapeutic results. Therefore, this study was designed
using forty T. canis-infected albino mice (1000 eggs/mouse, orally) and a control group (GI) of ten healthy mice. The infected
groups were GII: infected non-treated (control group), GIII: albendazole-treated (100 mg/kg/d once orally for 5 successive
days), GIV: MSCs-treated (3 x 106 MSCs in 0.1 mL of PBS via the tail vein) and GV: albendazole + MSCs-treated. Treatment
was commenced 6 weeks p.i. and the experiment was terminated four weeks after treatment. The brain tissue of each mouse
was subjected for histopathological, immunohistochemical studies (caspase-3, TGF-β), detection or T. canis DNA by real-time
PCR and gene expression the biomarkers of brain damage (S100B, GFAP) by RT-PCR. Moreover, homing of iron oxide-labelled
MSCs in brain tissues was assessed by Prussian blue stain. The brain tissues of GII showed numerous T. canis larvae, significant
congestion, thickening of arterioles, inflammatory infiltrate and gliosis associated with marked immunohistochemical
expression of TGF-β and caspase-3 as well as marked S100B and GFAP gene expression. Significant improvements of the
previous parameters and T. canis DNA were recorded in all the treated groups. However, the best results were obtained with
combined albendazole + MSCs therapy. Thus, MSCs could be considered in the treatment of chronic neurotoxocariasis.
Biography
Fatma shalan has completed her bachelor of medicine with excellent grade in 2006 from Faculty of Medicine, Menofia University. She has completed her .
in Medical Parasitology in 2012. Her thesis was about the relationship between IL-6 and some parasitic infections in hepatic patients in Menofia governorate. This
abstract is representative of her M.D. thesis.
Fatma Hamed Shalan et al., J Infect Dis Ther 2018, Volume 6
DOI: 10.4172/2332-0877-C5-050