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Volume 8, Issue 10 (Suppl) J Diabetes Metab
ISSN: 2155-6156 JDM, an open access journal Herbal Diabetes 2017
November 02-04, 2017
Page 12
conferenceseries.com
November 02-04, 2017 Bangkok, Thailand
23rd International Conference on
Herbal and Alternative Remedies for
Diabetes and Endocrine Disorders
The effect of Garcinia kola seed extract on the development of disease in an autoimmune mouse
model of type-1 diabetes
Type-1 Diabetes (T1D) is an insulin-dependent autoimmune disease characterized by T-cell mediated autoimmune
destruction of insulin-producing pancreatic β-cells. Garcinia Kola Heckel (GK), or bitter kola, is a medicinal plant
found in Central and Western Africa, which seeds’ extract (GKE) has been proposed to exhibit anti-inflammatory and
hypoglycemic properties. Considering that GKE has never been studied in the context of T1D and its enormous relevance
in folkloric medicine, we aimed to define whether GKE exhibits anti-diabetic properties and affects T-cells by its anticipated
anti-inflammatory action. Thus, it is hypothesized that GKE treatment would prevent development and reduce the severity
of T1D in an experimental mouse model, low-dose Streptozotocin (STZ)-induced autoimmune T1D, by affecting pathogenic
T-cells. Aqueous- and ethanol-based GKE were extracted and administered orally (via drinking water) to C57BL/6 male mice
in a dose of 100 mg/kg/day from 7 to 12 weeks of age. At 8 weeks of age, T1D was chemically induced by five consecutive
injections of 40 mg/kg STZ. Body weights and blood glucose levels were measured before STZ administration and bi-weekly
from day 8 until day 29 post first STZ injection. GKE mechanisms of action in the context of T-cells, such as the effect of
GKE on T-cell populations/subpopulations and T-cell function (proliferation assays and cytokine profiles), was studied as
well at two endpoints during disease development. The results showed that GKE treatment did not reduce body weights and
glycaemia; even a trend of elevated body weights and hyperglycemia levels was observed in GKE-treated mice. Surprisingly,
while decreasing T-cell populations/subpopulations, GKE exposure significantly increased T1D incidence in STZ-treated
mice. In conclusion, this study, as the first examination of the anti-diabetogenic potential of GKE in T1D, did not confirm its
potential in a reduction of hyperglycemia and prevention of T1D.
Biography
Marina Cetkovic-Cvrlje is a Professor of Immunology in the Department of Biology, Saint Cloud State University, USA. She has devoted her entire career to
autoimmune Type-1 Diabetes (T1D) research. Since joining SCSU, she has established a Laboratory for Immunology, providing research opportunities for
numerous undergraduate and graduate students to study effects of various environmental compounds on the development of autoimmune diabetes in murine
models of T1D. She has been teaching immunology, pathophysiology and public health controversies and has been a strong advocate for raising awareness about
effects of environmental compounds on T1D.
Marina Cetkovic-Cvrlje
St. Cloud State University, USA
Marina Cetkovic-Cvrlje, J Diabetes Metab 2017, 8:10 (Suppl)
DOI: 10.4172/2155-6156-C1-071