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Volume 8, Issue 2 (Suppl) J Blood Disord Transfus
ISSN: 2155-9864 JBDT, an open access journal Hematologists 2017
May 08-09, 2017
7th World Hematologists Congress
May 08-09, 2017 Barcelona, Spain
Intraoperative frozen section and imprint cytology of breast lesions: A comparative study
Shrinivas Kale, Smita Mulay, Anil Vare, S M Bindu and Meera Mahajan
MGM Medical College and Hospital, Aurangabad, India
Introduction: Frozen section is a vital technique used in diagnosis of breast lesions in agreement with imprint cytology. It helps
surgeons to take an immediate therapeutic decision, possibly sparing the patient a second operation and reducing hospitalization
cost. Frozen section in association imprint cytology has high degree of accuracy (varying from 94-98%).
Aim: Aim of this study is to evaluate usefulness of intraoperative frozen section for various breast lesions and to compare its diagnostic
yield with that of imprint cytology and paraffin sections
Setting: All surgically operated breast lesions were submitted intraoperatively to the histopathology and cytology department, MGM
Medical College and Hospital, Aurangabad during a period of July 2014 to June 2016 (23 months)
Subjects: 50 Indian female patients admitted to surgery ward at MGM Medical College and Hospital, Aurangabad because of breast
lesion and who had subsequent excision of their breast lesion.
Materials & Method: Touch imprint cytology smears were made from cut surface of specimen submitted followed by staining with
rapid PAP and subsequent frozen sections were made using Yorco Cryostat. The frozen sections were stained immediately with rapid
H & E. Remaining specimens were submitted for gold standard paraffin sections.
Results: There were 35 benign and 15 malignant lesions. There were two cases of significant discrepancies between frozen section,
imprint cytology and gold standard histopathology.
Conclusion: Our finding suggests that frozen section in agreement with imprint cytology gives correct diagnosis in 96% of cases
examined. It is very useful in evaluating margins also. Imprint cytology is cost effective over frozen section. Frozen section has few
demerits like freezing artifacts. Correlation with clinical, radiological and gross findings is necessary. Permanent paraffin sections
however are still a gold standard.
J Blood Disord Transfus 2017, 8:2(Suppl)
Vitamin D deficiency and its effect on hematopoietic stem cell transplant recipient
Hawazen Alsaedi
King Faisal Specialist Hospital and Research Centre, Saudi Arabia
Vitamin D plays an important role in calciummetabolism and homeostasis. In addition to its role in calciummetabolism, vitamin
D plays an important role as immunomodulator. Vitamin D receptor (VDR) is expressed on many cells including T, B and
natural killer (NK). Vitamin D modulates both innate and adaptive immune response. Recently, vitamin D deficiency is linked to
cancer, autoimmune diseases and graft versus host disease (GVHD). This study investigated the effect of vitamin D deficiency in
104 children who underwent hematopoietic stem cell transplant (HSCT) from 2012 to 2015. Vitamin D level was measured prior
to HSCT and serial measurements after HSCT on days 30, 60, 100 and 365 days. We observed that low vitamin level below 30 ng/
ml is strongly associated with increased risk of relapse and decrease survival at day 100-post hematopoietic stem cell transplantation
(HSCT) p=0.04. Also, we observed a strong correlation between low vitamin D level and infections including fungal and CMV
reactivation post HSCT p=0.004. To conclude, vitamin D plays an important role as immunomodulator; its deficiency may impact
the outcome of patient undergoing HSCT. Optimizing nutritional supplementation is very important before transplantation and
continues after HSCT.