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.com
Volume 7, Issue 4(Suppl)
J Gastrointest Dig Syst, an open access journal
ISSN: 2161-069X
Gastro Congress 2017
September 11-12, 2017
September 11-12, 2017 | Paris, France
12
th
Euro-Global Gastroenterology Conference
Hepatoprotective effect of glycyrrhizin and omega-3 fatty acids on nuclear factor-κb pathway in thioacetamide-
induced fibrosis in rats
Laila A EISSA, Nada F Abo El Magd, Amro El-Karef
and
Mamdouh M El-Shishtawy
Mansoura University, Egypt
N
uclear Factor Kappa B (NF-κB) is a key transcriptional regulator that plays important roles in the pathogenesis of hepatic
inflammation and fibrosis in chronic liver diseases with subsequent development of hepatocellular carcinoma. NF-kB activation
leads to production of pro-inflammatory and fibrogenic cytokines. Glycyrrhizin (GL) has been reported to suppress liver fibrosis
and cirrhosis. Omega-3 fatty acids (ω-3) have anti-inflammatory effects and they have been reported to decrease hepatic injury and
steatosis with subsequent fibrosis in Thioacetamide (TAA) fibrotic model.
Aim of the study:
To investigate the effects of GL and ω-3 alone and in combination on liver inflammation and fibrosis in rats and to
clarify the role of these natural compounds on NF-κB pathway.
Materials & Methods:
50 male Wistar rats randomized to 5 groups: Control group and 4 group received TAA 200 mg/kg i.p. twice
weekly for 8 weeks. TAA group, (TAA + GL) group (received GL 25 mg/kg/day by oral tube dissolved in distilled water started
with TAA), (TAA + ω-3) group (received ω-3 150 mg/kg/day by oral tube started with TAA), (TAA + GL + ω-3) group (received
similar but combined doses of both natural compounds with TAA). All groups were investigated for the effects of GL and ω-3 by the
assessment of ALT and AST activities, total bilirubin, albumin and total protein levels and liver MDA level by spectrophotometric
analysis, liver NF-κB level by ELISA and immunohistochemistry as well as histopathological analysis of the extent of liver fibrosis and
necroinflammatory activity.
Results:
TAA caused liver injury indicated by significant increase in serumALT and AST activities, total bilirubin level (P<0.005) with
significant decrease in serum albumin and total proteins levels (P<0.005). These results were also confirmed histopathologically by
the significant increase of the necroinflammatory scores (P<0.005) and extent of liver fibrosis. While GL, ω-3 and their combination
protected the liver from TAA hepatotoxic effects as they significantly decrease serum AST activity and total bilirubin level, also
they significantly increase serum albumin and total protein levels. The hepatoprotective effect of GL, ω-3 and their combination
also confirmed by histopathological analysis as they significantly reduced the necroinflammatory scores and the extent of fibrosis.
TAA caused significant increase in lipid peroxidation by increase liver MDA level (P<0.005). GL, ω-3 and their combination
decrease significantly liver MDA level (P<0.005). We also found that TAA has been increased NF-κB level in liver tissue (P<0.005),
while GL, ω-3 and their combination significantly decreased liver NF-ƙB level (P<0.005) and its tissue expression as detected by
immunohistochemistry.
Conclusion:
These results suggested that oxidative stress has an important role in the development of liver fibrosis mediated by
the regulatory role of NF-κB. Furthermore, glycyrrhizin and omega-3 fatty acids, alone and in combination have potent anti-
inflammatory, anti-oxidant and anti-fibrotic effects.
Biography
Laila A EISSA works for the Department of Biochemistry as faculty of Pharmacy in the University of Mansoura, Egypt. She has published nearly 30 articles in varied
journals.
lailaeissa2002@yahoo.comLaila A EISSA et al., J Gastrointest Dig Syst 2017, 7:4(Suppl)
DOI: 10.4172/2161-069X-C1-052