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Volume 7, Issue 5 (Suppl) J Forensic Res 2016
ISSN: 2157-7145, JFR an open access journal Forensic Research 2016
October 31-November 02, 2016
October 31-November 02, 2016 San Francisco, USA
5th International Conference on
Forensic Research & Technology
Investigation of polymorphic variants of SLC6A4, Tph-1 and Tph-2 genes in completed suicide
Duygu Yavuz1 , Ozge Cumaogullari1 , Nevriye Temel Yilmaz2, Nergis Canturk1, Bedriye Oncu1, Hilal Ozdag1 and N Lale Satiroglu Tufan1
1Ankara University, Turkey
2Forensic Medicine Institution, Turkey
Suicide is a big health problem in the world. Studies have shown that suicidal behavior has psychiatric, sociological, economic and
biological causes. In this thesis project, possible relationship between suicidal behavior and genetic structure was analyzed. Studies
in the literature have shown that serotonergic system affects the behaviors of people. Hence, polymorphic variant of rs1800532,
rs7305115, rs6355, rs1386494 regarding TPH-1, TPH-2 and SLC6A4 genes having important roles in serotonin production and
degradation pathways were selected for analysis. 100 completed suicide samples and 100 healthy control samples were included in
this study. Age (18-65) and the gender distribution were selected to be similar in suicide and control groups. Absence of psychiatric
disorder history other than depression, schizophrenia, bipolar mood disorder, mental retardation, chronic illness, drug and alcohol
addiction were noted in sample selection. DNA isolation was made from blood samples and then polymorphic variants, rs6355,
rs1800532, rs7305115, rs1386494, of TPH-1, TPH-2 and SLC6A4 genes were analyzed by KASP (Competitive Allele Specific PCR)
method. No significant difference in gender and age distribution was observed between suicide and control groups. Our results
showed that genotype and individual allele frequencies of polymorphic variants (rs1800532, rs7305115, rs6355, rs1386494) were not
significantly different in two groups. In conclusion, no association between TPH-1, TPH-2 and SLC6A4 gene polymorphisms was
observed in completed suicide samples.
Biography
Duygu Yavuz received her undergraduate degree in Biology at Hacettepe University in Ankara, Turkey. She is studying for her Master’s degree in Forensic Biology
at Forensic Science Institute, Ankara University. She is expected to receive the degree at the end of July. This study is her thesis project.
Duygu Yavuz et al., J Forensic Res 2016, 7:5(Suppl)