forensic-research-2016_scientifictracks-workshop

14 / 29

Page 38
Notes:
conferenceseries.com
Volume 7, Issue 5 (Suppl) J Forensic Res 2016
ISSN: 2157-7145, JFR an open access journal Forensic Research 2016
October 31-November 02, 2016
October 31-November 02, 2016 San Francisco, USA
5th International Conference on
Forensic Research & Technology
Isotopic quantitative NMR as a tool for authentication and traceability of drugs
Pierrick Nun
University of Nantes, France
Isotopic quantitative NMR (iqNMR) has been successfully applied to 2H since the 80’s (SNIF-NMR). Recently, EBSI team has been
able to extend it to 13C.1 It is now possible to measure with a precision as low as 1‰, the 13C/12C ratio on each carbon (δ13Ci), giving
a lot more information than the global 13C/12C ratio on the whole molecule (δ13Cg) obtained from isotope ratio measurement by
mass spectrometry (irm-MS). Indeed, only knowing the mean δ13Cg could be misleading. If the measured value is null, is it because
there is no fractionation or because there is counteractive normal and inverse isotopic effects on different positions? Quantitative 13C
NMR has already been successfully applied to a range of molecules including glucose, vanillin, paracetamol and aspirin and used to
show the position-dependent isotopic fractionation occurring during reactions or purifications. We developed new applications of
this powerful technique. In environmental studies, 13C iqNMR can be used to study the fate of pollutants. As an example, oxidation
of a fuel additive, Methyl tert-butyl ether has been done. MTBE was oxidized by potassium permanganate and the eventuality of a
position-specific fractionation has been investigated. In the actual debate around the origin of Tramadol, natural or anthropogenic,
mean values cannot give a clear answer and 13C iqNMR can help to establish a possible biosynthetic pathway. The isotopic fingerprint
could provide a unique tool for the authentication of drugs, depending of their synthesis, manufacturer or the origin of reactants.
Biography
Pierrick Nun has completed his PhD in 2009 from Montpellier University, France, where he worked on the application of alternative methodologies as
mechanochemistry in organic solvent-free synthesis. After Post-doctoral positions at St. Andrews University, Scotland, on gold catalysis and University of Caen,
France, on phosphine-boranes reactivity, he was appointed as an Assistant Professor in Nantes in 2012. He is currently working on applications of iqNMR in
environmental and pharmaceutical sciences and has published 27 papers and book chapters in peer-reviewed journals.
Pierrick Nun, J Forensic Res 2016, 7:5(Suppl)