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conferenceseries.com
October 27-29, 2016 Rome, Italy
15th International Conference on
Food Processing & Technology
Volume 7, Issue 12 (Suppl) J Food Process Technol 2016
ISSN: 2157-7110 JFPT, an open access journal Food Technology 2016
October 27-29, 2016
Characterization and classification of apple cultivars based on triterpenoids acids, phenolic constituents and
bioactive properties
Elisabeta-Irina Geana1 and Alexandru Ciocarlan2
1National R&D Institute for Cryogenics and Isotopic Technologies, Romania
2Academy of Sciences of Moldova, Moldova
Apple (Malus domestica Borkh.), a popular and widely cultivated fruit world-wide, contains active phytochemicals including
phenolic compounds and triterpenoids responsible for their health benefits. Here we report the results obtained for the
concentration of two major triterpenes (oleanolic and ursolic acids), phenolic, flavan-3-ols, flavonoids and bioactive properties of
different apple cultivars from different geographical regions and classification using chemometric analysis. Quantification of bioactive
compounds from apples was carried out by HPLC-PDA after an appropriate extraction method. Total polyphenols, total flavonoids
and antioxidant activity were recorder by spectrophotometric measurements. Our results demonstrated that the investigated bioactive
compounds and bioactive properties vary considerably depending on the apple cultivars. Catechin, epicatechin, chlorogenic acid and
rutin were the most important identified phenolic acids in apples (flesh and peel), while ursolic and oleanolic acids were abundant
in apple peel. There were significant linear correlations between phenolic content and antioxidant activity of extracts in the reaction
with DPPH• (1, 1-diphenyl-2-picrylhydrazyl). A linear discriminant analysis model was constructed to classify apples according
to the cultivar and to distinguish between different sample locations. Therefore, the present methodology based on apple active
phytochemicals fingerprinting and bioactive properties in conjunction with a comprehensive database and chemometric methods
presents a high potential for apple classification.
Biography
Elisabeta-Irina Geana has completed her PhD in 2015 at Bucharest University. Her main current interests are identification and quantification of essential active
principles like phenolic compounds, organic acids, amino acids, terpenes, micro and macronutrients in different food matrices (wine, honey, fruits, plants, organic
products, functional foods) by highlighting key biomarkers used in authentication, using the main instrumental analytical (HPLC, UV-VIS, ICP-MS). She has
published 16 ISI articles, 10 of them as first author and has 145 citations.
Elisabeta-Irina Geana et al., J Food Process Technol 2016, 7:12 (Suppl)