food-technology-2016_posters-accepted-abstracts

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conferenceseries

.com

October 27-29, 2016 Rome, Italy

International Conference on

Food Processing & Technology

Volume 7, Issue 12 (Suppl)

J Food Process Technol 2016

ISSN: 2157-7110 JFPT, an open access journal

Food Technology 2016

October 27-29, 2016

Hye Soon Kang et al., J Food Process Technol 2016, 7:12 (Suppl)

Simultaneous analysis of diuretic drugs in food and dietary supplements using LC/PDA and LC/MS/MS

Hye Soon Kang, Hyung-Il Kim, Moo-Song Lim, Joong-Hoon Cho, Chul-Joo Lim

and

Sun-Ok Choi

Gyeongin Regional Food and Drug Safety, South Korea

iuretic drugs such as chlorothiazide, hydrochlorothiazide, hydroflumethiazide, trichloromethiazide and methylchlothiazide are

illegal to be included in food and dietary supplements since abuse intake of the drugs via food without medical prescription

can be caused life-threatening side effects. Governmental agencies of food and drug safety invest their efforts to screen out food and

dietary supplements illegally containing diuretic drugs. We developed an accurate, simple, rapid and simultaneous analysis method

of five thiazide diuretic durgs in food and dietary supplements using liquid chromatography coupled with photodiode array detection

(LC/PDA). The developed method was fully validated and showed good results with respect to specificity, linearity (r

>0.99), limit of

detection (0.1µg/mL), limit of quantification (0.4µg/mL), precision (RSD<3.3%), recovery (92.1~106.4%), and reproducibility. It also

satisfied all standards suggested by AOAC for the analysis of diuretic drugs in food and food supplements. To confirm the detected anti-

obesity drugs in food and dietary supplements, we also developed a qualitative analysis method by Liquid chromatography coupled

with mass spectrometry (LC/MS). Ninety foods and dietary supplements purchased by internet and local market in Korea were tested

with the developed method. Diuretic drugs were not detected in all samples. LC/PDA and LC/MS methods described in this study

were simple, rapid and reliable; the methods may be suitable for a rapid and sensitive analysis of chlorothiazide, hydrochlorothiazide,

hydroflumethiazide, trichloromethiazide and methylchlothiazide simultaneously in food and dietary supplements.

Biography

Hye Soon Kang is a Scientific Officer in Gyeongin Regional Food and Drug Safety of Ministry of Food and Drug Safety in Korea.