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conferenceseries
.com
October 27-29, 2016 Rome, Italy
15
th
International Conference on
Food Processing & Technology
Volume 7, Issue 12 (Suppl)
J Food Process Technol 2016
ISSN: 2157-7110 JFPT, an open access journal
Food Technology 2016
October 27-29, 2016
Hye Soon Kang et al., J Food Process Technol 2016, 7:12 (Suppl)
http://dx.doi.org/10.4172/2157-7110.C1.057Simultaneous analysis of diuretic drugs in food and dietary supplements using LC/PDA and LC/MS/MS
Hye Soon Kang, Hyung-Il Kim, Moo-Song Lim, Joong-Hoon Cho, Chul-Joo Lim
and
Sun-Ok Choi
Gyeongin Regional Food and Drug Safety, South Korea
D
iuretic drugs such as chlorothiazide, hydrochlorothiazide, hydroflumethiazide, trichloromethiazide and methylchlothiazide are
illegal to be included in food and dietary supplements since abuse intake of the drugs via food without medical prescription
can be caused life-threatening side effects. Governmental agencies of food and drug safety invest their efforts to screen out food and
dietary supplements illegally containing diuretic drugs. We developed an accurate, simple, rapid and simultaneous analysis method
of five thiazide diuretic durgs in food and dietary supplements using liquid chromatography coupled with photodiode array detection
(LC/PDA). The developed method was fully validated and showed good results with respect to specificity, linearity (r
2
>0.99), limit of
detection (0.1µg/mL), limit of quantification (0.4µg/mL), precision (RSD<3.3%), recovery (92.1~106.4%), and reproducibility. It also
satisfied all standards suggested by AOAC for the analysis of diuretic drugs in food and food supplements. To confirm the detected anti-
obesity drugs in food and dietary supplements, we also developed a qualitative analysis method by Liquid chromatography coupled
with mass spectrometry (LC/MS). Ninety foods and dietary supplements purchased by internet and local market in Korea were tested
with the developed method. Diuretic drugs were not detected in all samples. LC/PDA and LC/MS methods described in this study
were simple, rapid and reliable; the methods may be suitable for a rapid and sensitive analysis of chlorothiazide, hydrochlorothiazide,
hydroflumethiazide, trichloromethiazide and methylchlothiazide simultaneously in food and dietary supplements.
Biography
Hye Soon Kang is a Scientific Officer in Gyeongin Regional Food and Drug Safety of Ministry of Food and Drug Safety in Korea.
erclo@korea.ac.kr