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Pharmaceutica Analytica Acta | Volume: 09
September 20-22, 2018 Prague, Czech Republic
Pharmaceutics & Drug Delivery Systems
17
th
Annual Congress on
Baicalein attenuates LPS-induced oxidative stress and inflammation, might be via CaMKII inhibition
Mary X Zhang
1
, Jiming Ye
1
, Dominic Ng
2
, Ross Vlahos
1
, Steve Bozinovski
1
, Jun Xu
3
and
Charlie C Xue
1
1
RMIT University, Australia
2
University of Queensland, Australia
3
Sun Yat-Sen University, China
O
xidative stress and inflammation are two major contributing factors to most chronic diseases including cancer, diabetes,
cardiovascular, neurological and pulmonary diseases. Baicalein, a flavonoid bioactive compound from Scutellaria baicalensis
Georgi (also known Scutellaria Radix orHuangQin), has demonstrated antioxidant and anti-inflammatory potentials. However, the in-
depth mechanistic studies on this compound with protective effects remain to be discovered. This study on lipopolysaccharide (LPS)-
induced reactive oxygen species (ROS) in macrophages showed that pre-treatment with baicalein significantly attenuated oxidative
stress by reducing the ROS levels in a dose-dependent manner (0.1 µM, 0.33 µM, 1 µM, 3.3 µM and 10 µM). The antioxidant effects of
baicalein were 100 times stronger than N-Acetylcysteine (NAC), a commercial antioxidant agent. Furthermore, the antioxidant effects
of baicalein showed the same inhibitory pattern with KN-93, a specific inhibitor of CaMKII, in the LPS-induced ROS production
in vitro. Moreover, pre-treatment of baicalein also significantly decreases LPS-induced inflammation in macrophages by reducing
the pro-inflammatory mediators, including IL-6, monocyte chemoattractant protein 1 (MCP-1), macrophage inflammatory protein
-1(MIP-1), regulated on activation in normal T-cell expressed and secreted (RANTES) and tumor necrosis factor (TNF α) in a
dose-dependent manner as well. We are currently identifying if CaMKII (Ca
2+
/calmodulin-dependent protein kinase II) would be a
potential primary target by baicalein with utilizing small-molecule affinity purification, and if the decreased oxidative stress which
could lead to the reduced inflammation by baicalein’s inhibition of CaMKII involves the attenuation of p38MAPK and JNK signaling
pathway for LPS-induced cytokine production by macrophage.
mary.zhang@rmit.edu.auPharm Anal Acta 2018, Volume: 9
DOI: 10.4172/2153-2435-C2-037