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conferenceseries
.com
Euro Pathology 2018 | Hematologic Oncology 2018
June 20-21, 2018
Volume 8
Journal of Clinical & Experimental Pathology
ISSN : 2161-0681
15
th
EUROPEAN PATHOLOGY CONGRESS
&
LEUKEMIA AND HEMATOLOGIC ONCOLOGY
June 20-21, 2018 | Paris, France
14
th
International Conference on
JOINT EVENT
Antimicrobial activity of lactic acid bacteria isolated from camel raw and fermented (Garis) milk against
pathogenic bacteria isolated from urinary tract infected patients, Sudan
Nahed Adam Abdalla Jebrel
1
, Asmaa B Ibrahim
1
, Hisham N Altayb
1
and Zeinab A M Elgadi
2
1
Sudan University of Science and Technology, Sudan
2
Veterinary Research Institute, Sudan
T
his was a cross-sectional study carried out to isolate, identify and test the antimicrobial activity of lactobacillus species
against pathogenic bacteria. Three strains of Lactobacillus species (
Lactobacillus acidipiscis, Lactobacillus rhamanosus
and
Lactobacillus lactis
subsp.
hordinae
) were isolated from camel milk, and 30 bacterial species were isolated from urinary tract
infected patients by using various cultured media. Strains isolated were characterized by phenotypic, physiological and biochemical
properties. Identification of Lactobacilli species was confirmed by sequencing of 16S rRNA gene. Well diffusion method was used
for antimicrobial activity of Lactobacillus species and commonly used antibiotic, against pathogenic bacteria. Results showed
that Escherichia coli was the most frequently isolated urinary pathogen 9 (30%) followed by
Klebsiella pneumoniae
8 (26.7%),
Enterococcus faecalis
6 (20%),
Staphylococcus aureus
5 (16.7%),
Pseudomonas aeruginosa
1 (3.3%) and
Proteus mirabilis
1 (3.3%).
Most of the isolated organisms were resistant to co-trimoxazole 24 (80%), norfloxacin 12 (40%), ciprofloxacin, nitrofurantoin 10
(33.3%) and chloramphenicol 10%. Four (13.3%) isolates of pathogenic bacteria were susceptible to
Lactobacillus lactis
subsp.
hordinae activity, followed by 2 (6.9%) isolates susceptible to
Lactobacillus rhamanosus
and 1 (3.3%) susceptible Lactobacillus
acidipiscis,
Lactobacillus lactis
subsp. hordinae could be used for the treatment and prevention of multidrug resistant urinary tract
infected bacteria.
Biography
Nahed Adam Abdalla Jebrel has completed her MA from National University of Sudan and BSc in Histopathology and Cytology from Sudan International University.
She has published one paper in a reputed journal and has expertise in DNA sequencing and sequences analysis, NGS sequencing and data analysis, bioinformatics
techniques (Modeling, Mutation analysis, immunoinformatics (BLAST and NCBI)), PCR technique and DNA sequencing and sequences analysis.
nahedadam7@gmail.comNahed Adam Abdalla Jebrel et al., J Clin Exp Pathol 2018, Volume 8
DOI: 10.4172/2161-0681-C1-046