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Volume 7, Issue 2 (Suppl)
J Ecosyst Ecography, an open access journal
ISSN:2157-7625
September 18-20, 2017
September 18-20, 2017 Toronto, Canada
Joint Conference
International Conference on
International Conference on
Environmental Microbiology and Microbial Ecology
&
Ecology and Ecosystems
Diel cycle of the tropical air microbiome
Elena S Gusareva
Singapore Centre for Environmental Life Sciences Engineering, Singapore
O
ur understanding of airborne microbial communities including their sources, functions, microbial interplay and ecology is
limited despite its relevance for human health, environmental and ecosystems functioning. Few attempts have been made to
study air microbial ecosystems to date. Tropical environments, distinct from temperate northern hemisphere systems studied so
far, have specific physical/chemical features that impact the structure, function and ecology of the microbial components of their
air ecosystems. Moreover, a highly urbanized ecosystem with its unique built environment and extensively processed indoor air, as
encountered in Singapore, likely harbors defined yet unknown microbial communities.
To comprehensively describe air-borne microbial ecosystem of Singapore, triplicates air samples were collected within 24 hours for 5
consecutive days in four independent experiments. We used filter-based high volume air sampling instruments to generate samples
used for subsequent DNA extraction. A cultivation-free metagenomics next generation sequencing (NGS) was adopted to identify
microbial taxa to species level. The air-borne microbial community was found to be remarkably stable with recurrence intervals of
high microbial abundance in the dark hours and relatively low abundance in the light hours. Air samples were dominated by DNA
of basidiomycota fungi and less frequently of Ascomycota fungi phylum. The microbial air ecosystem was mostly modulated by day/
night Biorhythms, temperature fluctuations and rain events. This is one of the highest-resolution researches currently undertaken on
any air microbiome and its ecology, worldwide.
Biography
Elena S Gusareva has evolved to be a researcher with a broad knowledge and experience in different aspects of biomedical science and biostatistics. Since joining
the team of Prof. Schuster in 2016, she has been involved in a relatively new research field and project on the air microbiome aiming to study microbial diversity and
ecology of urban air. So far, the results of the research efforts are remarkable and will certainly generate a series of downstream benefits, including improvements
to public health, building design and management.
egusareva@ntu.edu.sgElena S Gusareva, J Ecosyst Ecography 2017, 7:2 (Suppl)
DOI: 10.4172/2157-7625-C1-029