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Volume 8 Journal of Environmental & Analytical Toxicology Environment and Health Congress 2018
July 11-12, 2018
July 11-12, 2018 Sydney, Australia
World Congress on
Environmental Toxicology and Health
Effect of SF-1 in maternal cadmium exposure inhibit testosterone synthesis on male offspring
Yuanyuan Huang, Jingwen Zeng, Jianlin Zhu, Yuchen Li, Wenchang Zhang and Jin Liu
Fujian Medical University, China
Background: Toxic effects of environmental reproductive toxicant cadmium on the testis have been confirmed and the toxicity
of maternal cadmium exposure on the testis of offspring has become more important topic currently.
Objective & Design: SD female rats were treated with intra-gastric administration of cadmium (0, 0.5, 2, 8 mg/kg CdCl2)
during pregnancy. After contamination, serum and testis tissue were collected from the male first (F1) and second (F2) filial
generations, respectively, at PND21 and PND56. The growth and development of the testis and the serum level of sex hormone
were observed. The expression of steroidogenic factors in Leydig cells was detected. The roles of SF-1 regulating the expression
of steroidogenic factors in hormone synthesis and secretion and its epigenetic mechanism were explored.
Result: Maternal exposure to cadmium during pregnancy caused a significant decrease in serum testosterone level in male F1
offspring, an obvious inhibition in the expression of SF-1 and corticosteroid-related proteins. Epigenetics showed abnormal
expression of miR-10b-5p and miR-328a-5p, which regulated StAR and SF-1 (NR5A1), respectively, confirmed by luciferase
report gene assay. SF-1 mRNA expression, corticosteroid-related protein expression and serum testosterone level increased
significantly in the testis of F2 offspring.
Conclusion: Maternal exposure to cadmiumcan affect testicular development of male offspring. SF-1may regulate the expression
of steroidogenic factors, which play an important role in maternal exposure to cadmium inhibiting testosterone in offspring.
Biography
Yuanyuan Huang is pursuing her Master’s degree in Fujian Medical University, China. Her specialty is labor hygiene and environmental hygiene and her research
direction is environmental molecular toxicology.
Yuanyuan Huang et al., J Environ Anal Toxicol 2018, Volume 8
DOI: 10.4172/2161-0525-C1-011