21
conferenceseries LLC Ltd
3
rd
International Conference on
3
rd
International Conference on
Ecology, Ecosystem and Conservation Biology
Microbial Ecology & Eco Systems
&
March 18-19, 2019 | Chicago, USA
Find More Information @
https://conferenceseries.com/america/MARCH 2019 Conference Series LLC Ltd
JOURNAL OF ECOSYSTEM& ECOGRAPHY 2019, VOLUME 9 | DOI: 10.4172/2157-7625-C1-044
SCIENTIFIC TRACKS
|
DAY 1
Phosphoproteome
analysis of
Trichoderma
reesei
reveals a post
translation regulation
of secreted glycosyl
hydrolases
Liliane FC Ribeiro, Wellington Pedersoli, Renato
P Graciano, Amanda CC Antonieto, Maira P
Martins, Fausto Almeida, Rafael Silva-Rocha,
Antonio Rossi Filho,
and
Roberto N Silva
University of Sao Paulo, Brazil
T
richoderma reesei
is
the major producer of
cellulases and hemicellulases
and the regulation of
these enzymes expression
is not fully understood.
Phosphorylation events
are known to be involved
in the regulation of various
cellular processes, including
metabolism, transcription,
translation regulation,
protein degradation,
homeostasis, signaling,
and protein secretion,
among others. In
T. reesei
,
protein phosphorylation
pattern changes in a carbon
dependent way. In order to
have a broad understanding
of the phosphorylation events
and how it affects different
cellular functions, once T.
reesei is grown in sugarcane
bagasse, the main biomass
for bioethanol production
in Brazil, we conducted an
experiment in which we
identified phosphorylated
proteins by LC-MS/MS.
CBHI was identified as a
phosphorylated protein in the
intracellular protein extract,
with five phosphorylation sites.
In order to test the importance
of the phosphorylation for this
enzyme, CBHI was purified
from the secretome of
T. reesei
and
dephosphorylated in
order to detect any enzymatic
activity difference caused by
phosphorylation. CBHI activity
was about 60% less after
dephosphorylation. The broad
phosphorylation pattern that
happens in
T. reesei
when it is
cultivated in sugarcane bagasse
provides novel information
about several proteins and
phosphosites not previously
described. This work shows
for the first time that some
secreted glycosyl hydrolases
are phosphorylated in the
condition tested and suggests
that CBHI activity is modulated
by phosphorylation.
Biography
Liliane Ribeiro has completed
her PhD at the age of 30 years
from University of Sao Paulo
and Postdoctoral studies
from University of Maryland
working with identification
of new targets of kinases.
Currently she is a Post-doc
fellow at University of Sao
Paulo, where she studies
phosphorylation events
related to the carbon source
offered to the fungus. She
has published 12 papers
in reputed journals in the
microbiology field so far.
lilianebqi@usp.br