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Volume 7, Issue 6 (Suppl)

J Alzheimers Dis Parkinsonism, an open access journal

ISSN: 2161-0460

Dementia 2017

October 16-18, 2017

ALZHEIMER’S DISEASE & DEMENTIA

October 16-18, 2017 | Rome, Italy

International Conference on

J Alzheimers Dis Parkinsonism 2017, 7:6(Suppl)

DOI: 10.4172/2161-0460-C1-034

Effect of magnetic tacrine-loaded chitosan nanoparticles on spatial learning, memory, amyloid

precursor protein and seladin-1 expression in the hippocampus of streptozotocin-exposed rats

Golamreza Hassanzadeh

Tehran University of Medical Sciences, Iran

lzheimer's disease (AD) is a progressive neurodegenerative disease characterized by memory and cognitive dysfunction

due to neuronal cell loss in higher brain centers. Senile plaques containing amyloid β (Aβ) are associated with this disease

as well as a reduction in cholinergic neuron numbers. Tacrine is a reversible cholinesterase inhibitor in clinical use to treat

moderate forms of AD. Chitosan nanoparticles represent an effective systemic delivery system for drugs. The application of

tacrine-loaded chitosan nanoparticles has been shown to selectively increase tacrine concentrations in the brain tissue. In this

study, we comparedmagnetic and non-magnetic tacrine-loaded chitosan nanoparticles for their bioactivity and neuroprotective

potency in streptozotocin (stz)-induced neurodegeneration, an accepted animal model for AD. Male rats received a single

injection of stz via an implanted cannula into the lateral brain ventricle. Tacrine (tac)-loaded chitosan nanoparticles were

delivered into the tail vein. Spatial learning and memory were analyzed using the Morris water maze task. Amyloid precursor

protein gene (APP) and seladin-1 gene expression were studied in the hippocampus by real time-PCR. Tac-loaded non-

magnetic and tac-loaded magnetic chitosan nanoparticles improved spatial learning and memory after stz treatment with

magnetic nanoparticles being most effective. Similarly, tac-loaded chitosan nanoparticles increased seladin-1 and reduced APP

gene expression. Again, magnetic nanoparticles were more effective. These data reveal that tac-loaded non magnetic and tac-

loaded magnetic chitosan nanoparticles to a higher extent improve brain deficits related to stz application. We conclude that

the magnetic target drug delivery system is a promising therapeutic strategy to protect AD-related degenerating in the CNS.