clinical-gastroenterology-digestive-diseases2017-posters

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Volume 7, Issue 6 (Suppl) J Gastrointest Dig Syst, an open access journal
ISSN: 2161-069X
Page 71
December 07-08, 2017 Madrid, Spain
&
13th International Conference on Clinical Gastroenterology & Hepatology
2nd International Conference on Digestive Diseases
CO-ORGANIZED EVENT
The impact of toll-like receptor 9 polymorphisms on hepatitis B virus clearance
Hajar Chihab1,2, F-Z Jadid1, A El Habazi2, M Chair2, W Badre3, M Tahiri3, I Chemin4, P Pineau1, S Ezzikouri1 and S Benjelloun1
1Institut Pasteur du Maroc, Maroc
2Université Chouaib Doukkali, Maroc
3CHU Ibn Rochd, Morocco
4Université Claude Bernard, France
Statement of the Problem: Hepatitis B infection remains a serious public health problem in the world. In connection with
poorly defined defects affecting their immune competence, patients chronically infected with hepatitis B virus (HBV) cannot
clear the virus. The outcome of infection depends primarily on the interaction between the virus and selected effectors of host
immunity. Toll-like receptor 9 (TLR9) plays a crucial role in innate immunity against viral infections through detection of
intra-cytoplasmic dsDNA. Defects in this system may result, therefore, in attenuated responses against HBV. Recent research
has focused on the possibility of targeting the defects in TLR9 pathway as a novel approach for anti-HBV treatment. Our study
aimed to assess the impact of both TLR9 rs5743836 and rs187084 polymorphisms on spontaneous HBV clearance in Moroccan
patients.
Material/Methods In this study, 239 chronic HBV (CHB) patients and 134 spontaneously resolved HBV (SRB) individuals
were recruited and genotyped using a Taqman allelic discrimination assay.
Results: Remarkably, we observed dosage effect of both SNPs on viral loads. At rs5743836, AA, AG and GG genotypes were
significantly associated with a progressive increase of circulating HBVDNA whereas the inverse phenomenon was noticed with
AA, AG and GG at rs187084. By contrast, there was no consistent association between TLR9 polymorphisms and spontaneous
clearance or persistence of HBV.
Conclusion: To conclude, of Moroccan patients, no significant association of rs5743836 and rs187084 TLR9 polymorphisms
was observed with HBV natural clearance. Further studies on larger populations should shed light on the modulating effect of
TLR9 polymorphisms on HBV loads that remain a viral factor of paramount importance to predict HCC development.
J Gastrointest Dig Syst 2017, 7:6(Suppl)
DOI: 10.4172/2161-069X-C1-059