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conferenceseries.com
Volume 2 Environment Pollution and Climate Change
ISSN: 2573-458X Climate Change 2018 &
Global ENVITOX 2018
October 04-06, 2018
October 04-06, 2018
London, UK
16th Annual Meeting on
Environmental Toxicology and Biological Systems
&
5th World Conference on Climate Change
JOINT EVENT
Effects of aluminium salts and cyclic volatile methylsiloxanes on DNA damage and DNA repair in
immortalized non-transformed human breast epithelial cells
Abdullah Mohammed O Farasani1,2 and Darbre P D2
1Jazan University, Saudi Arabia
2University of Reading, UK
Dermal absorption of components of underarm cosmetics may be a contributory factor in breast cancer development.
Aluminium (Al) salts are added as the active antiperspirant agent, and cyclic volatile methylsiloxanes (cVMS) are
used for purposes of conditioning and spreading. Al has been measured in human breast tissue, breast cyst fluid, nipple
aspirate fluid and milk: Al levels in breast tissue have been recently reported to be a risk factor for breast cancer in young
women. cVMS have been measured in human milk. The objectives of this study were to investigate any genotoxic effects of
exposure to the antiperspirant salts Al chloride and Al chlorohydrate, and to the cVMS hexamethylcyclotrisiloxane (D3),
octamethylcyclotetrasiloxane (D4) and decamethylcyclopentasiloxane (D5) in immortalized non-transformed human breast
epithelial cells. All these compounds enabled a dose-dependent growth of the non-transformed cells in suspension culture,
which is an established marker of transformation. DNA damage was demonstrated using a comet assay. Long term (≥20 weeks)
exposure to these compounds also resulted in loss of expression (mRNA and protein) of the breast cancer susceptibility gene
BRCA1 which is a key gene in the repair of DNA in breast cells. Alterations to expression of other DNA repair genes at an
mRNA level will be presented. If these compounds can both damage DNA and compromise DNA repair systems, then there is
the potential for breast carcinogenesis.
Recent Publications
1. A and Darbre P D (2017) Exposure to cyclic volatile methylsiloxanes (cVMS) causes anchorage-independent growth and
reduction of BRCA1 in non-transformed human breast epithelial cells. Journal of Applied Toxicology 37(4):454–461.
2. Farasani A (2017) Importance of exome sequencing in the human diseases and medical genetics. J Genet Genomic Sci.
2:006.
3. Farasani A and Darbre P D (2015) Effect of aluminium chloride and aluminium chlorohydrate on DNA repair processes in
MCF10A immortalized non-transformed human breast epithelial cells. Journal of Inorganic Biochemistry 152(2015):186–9.
Biography
Abdullah Mohammed O Farasani received his PhD from Reading University, UK in 2016 for his work on genotoxicity of cosmetic chemicals in human breast epithelial cells.
He is currently working as the head of Genetic Unit in the MLT Department at Jazan University, Saudi Arabia.
Abdullah Mohammed O Farasani et al., Environ Pollut Climate Change 2018, Volume 2
DOI: 10.4172/2573-458X-C1-002