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Chromatography 2016
September 21-23, 2016
Volume 7, Issue 5(Suppl) J Chromatogr Sep Tech 2016
ISSN: 2157-7064 JCGST, an open access journal
conferenceseries .com
September 21-23, 2016 Amsterdam, Netherlands
World Congress on Chromatography
Lignocellulosic hydrolyzate characterization using anion exchange chromatography
Nico Anders, Harald Humann, Bernd Langhans and Antje Spieß
RWTH Aachen University, Germany
Hydrolyzates from lignocellulosic biomass are a complex mixture of soluble monomeric and oligomeric fragments. These
fragments are degradation products from all four natural polymers cellulose, hemicellulose, pectin and lignin. As the
polarity and size varies for all fragments depending on the origin, the analysis requires more than one analytical method and
a broad know how. Anion exchange chromatography (AEC) has a great potential as the measurement is based on the ability
of producing anions from soluble lignocellulosic biomass derived fragments. Thus, the aim of this study was to characterize
hydrolyzates from different biomasses for soluble biomass derived degradation products with AEC. Therefore, the parameters
column temperature (30°C to 50°C), eluent composition and chromatographic run time were investigated for separation of the
biomass derived degradation products and short chromatographic run time. The final method was set to a column temperature
of 40°C, an eluent flow of 1mL/min and an eluent consisting of sodium acetate and sodium hydroxide as well as ultrapure water.
Using the newly developed method a run time of 70 min could be realized for degradation products from all natural polymers.
Additionally, the limit of detection in the range of 0.014 mg/L for 2, 6-dimethoxyphenol and 21.9 mg/L for 4-methoxybenzyl
alcohol allows for a simultaneous determination of lignin derived compounds beside the high glucose concentrations. In
consequence AEC was used to characterize hydrolyzates from 17 lignocellulosic biomasses for soluble compounds derived
from cellulose, hemicellulose, pectin and lignin.
Biography
Nico Anders has been working in the field of analysis and renewables since 2009. He has obtained his PhD at the TU Braunschweig in the group of Prof. Dr. Vorlop
in Technical Chemistry. Since 2013, he is working as junior research group leader in the Aachener Verfahrenstechnik at the RWTH Aachen University. The research
interests of Nico Anders are analysis of lignocellulosic biomass, green analytical chemistry, conversion of lignocellulosic biomass and chromatographic separation.
Nico Anders et al., J Chromatogr Sep Tech 2016, 7:5(Suppl)