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Chromatography 2016
September 21-23, 2016
Volume 7, Issue 5(Suppl) J Chromatogr Sep Tech 2016
ISSN: 2157-7064 JCGST, an open access journal
conferenceseries .com
September 21-23, 2016 Amsterdam, Netherlands
World Congress on Chromatography
Preparation of tetraoxocalix [2]arence[2]triazine coated Fe3O4 @SiO2 magnetic nanoparticles and its
application in determination of PAHs in smokers urine
Shusheng Zhang, Yanhao Zhang, Huifang Du and Wenfen Zhang
Zhengzhou University, China
In the present paper, tetraazacalix[2]arene[2]triazine coated magnetic nanoparticles (TCFe3O4@SiO2) was prepared and
characterized. The performance using TCFe3O4@SiO2 as SPE sorbent was investigated using PAHs as probes. Under the
optimized SFP condition for PAHs, the urine samples (300 mL) were directly extracted, eluted, evaporated and re-dissolved
in 0.3 mL ACN, and then 20 uL was injected for HPLC separation and analysis. The recoveries were also tested and obtained
for 85% for Phe, 88% for Ant, 92% for Pyr, 96% for Chr and 93% for Bap by spiking each PAH at 5pg/ml. The each PAH
concentration level for the smokers was at 0.5-4.5 pg/ml, and the higher PAH concentration levels were found in the urines
who smoke more cigarettes. The PAH concentration levels for the heavy smokers (40 cig/d) were doubled those for the non-
smokers. The very low Bap concentration level at 0.4-0.9 pg/ml was also sensitively and accurately detected. The method
showed good extraction efficiency for PAHs due to tetraoxocalix[2]arence[2]triazine having benzene rings, which interacted
with PAHs based on π-π interaction. The SPE extraction is simple because of the use of the magnetic nanoparticles TCFe3O4@
SiO2. This SPE material can be widely used in the sample pretreatment.
Biography
Shusheng Zhang has completed his PhD from Zhengzhou University and Post-doctoral studies from Tasmania University of Australia. He is the Director of Center
of Advanced Analysis & Computational Science of Zhengzhou University. He has published more than 180 papers in reputed journals.
Shusheng Zhang et al., J Chromatogr Sep Tech 2016, 7:5(Suppl)