Volume 2, Issue 3(Suppl)
Oncol Cancer Case Rep
ISSN: 2471-8556 an open access journal
Page 50
Notes:
Cancer Therapy & Biomarkers 2016
December 05-07, 2016
conferenceseries
.com
CANCER THERAPY,
BIOMARKERS & CLINICAL RESEARCH
15
th
World Congress on
December 05-07, 2016 Philadelphia, USA
An
in vitro
evaluation of carmustine-loaded Nano-co-Plex for potential magnetically targeted intranasal
delivery to the brain for brain tumor management
Olufemi David Akilo
University of the Witwatersrand, South Africa
T
he aim of this work was to synthesize polyvinyl alcohol/polyethyleneimine/folate (Polyplex) complex coated Magnetite
(Nano-co-Plex) with superparamagnetic capability and targeting attributes loaded with carmustine (BCNU) for poten-
tial magnetically targeted delivery of BCNU to the brain following intranasal administration for brain tumor management.
This was achieved by co-precipitation reaction of Fe2
+
and Fe3
+
at high pH under N2, epoxidation of PVA and EDC/NHS
coupling reaction of Folate with PEI. Drug loading was carried out in the dark. The release study was carried out by dispers-
ing the dried BCNU-Nano-co-Plex in PBS pH 7.4 at 37°C. The formulation was characterized employing Fourier Transform
Infrared Spectroscopy (FTIR), Thermogravimetric Analysis (TGA), Transmission Electron Microscope (TEM), Zetasizer,
Superconducting quantum interference device (SQUID) and X-ray Diffraction (XRD) techniques. FTIR spectra confirmed
the synthesis of BCNU-loaded Polyplex coated Magnetite. The morphology, size and stability of the formulation showed
hexagonally shaped particles with average size of 45nm with zeta potential of +21mV and poly dispersity index (PDI) of 0.22.
XRD results further confirmed the crystalline nature of the formulation. The thermal analysis indicated that 1/3 of the total
weight of the formulation constituted the drug-loaded polymeric coating. The magnetic studies showed superparamagnetic
attribute of the formulation with high magnetization value. The loading capacity of the synthesized Nano-co-Plex was effi-
cient for BCNU. The release profiles indicated a sustainable release of BCNU, with up to 75% of drug released after 72 hours.
BCNU-loaded Nano-co-Plex was synthesized successfully.
Biography
Olufemi David Akilo has just completed his PhD in Pharmaceutics specializing in drug delivery systems at the Department of Pharmacy and Pharmacology,
University of the Witwatersrand, Johnnesburg South Africa. He currently works with Faculty of Health Sciences, University of the Witwatersrand University. He has
authored a book chapter, research paper in a reputed journal and has filed a patent with South Africa patent Agency. He has also presented papers in several local
and international conferences.
femiakilo@gmail.comOlufemi David Akilo, Oncol Cancer Case Rep 2016,2:3(Suppl)
http://dx.doi.org/10.4172/2471-8556.C1.002