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Journal of Gastrointestinal & Digestive System | ISSN: 2161-069X | Volume: 8
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October 29-30, 2018 | San Francisco, USA
International Conference on
Gastrointestinal Cancer and Therapeutics
4
th
World Congress on
Digestive & Metabolic Diseases
26
th
Annual Congress on
Cancer Science and Targeted Therapies
Physical tissue expansion for nanoscale investigation of clinical specimens
Objective:
In pathology, examination of cellular structures and molecular composition using diffraction-limited microscopy is
key to diagnosis. Recently, a new approach, Expansion Microscopy, was developed, enabling physical magnification and high
resolution imaging of cell lines and mouse brain sections with conventional optical microscopes, by embedding them in a dense
swellable polymer and adding water to swell the polymer after the enzymatic digestion of the proteins (Chen F et al., Science,
2015). The purpose of our study is to develop a pathology-optimized physical tissue expansion method for nanometer imaging
and investigation of clinical tissue samples and to analyze its utility in diagnostic pathology and research.
Methods:
We developed a pathology optimized physical tissue expansion methods called Expansion Pathology (ExPath),
which uses clinically optimized chemistry, labeling and imaging methodologies to expand and visualize both human FFPE and
frozen clinical samples, including previously stained/unstained, mounted/unmounted and whole tissue slide/tissue microarrays
sections, of a wide variety of fixed human tissue types and pathologies.
Results:
This ExPath protocol enabled expansion of human normal and cancer tissues (including gastrointestinal malignancies)
~4.5x in linear dimension and ~100x in volume, with a post-expansion measurement error of 3-7%. Physical tissue expansion
pushes theopticalmicroscopesbeyond their limits (currently250nminresolution), byenabling for thefirst time~70nmresolution
imaging of diverse biomolecules in intact tissue with an optical microscope. With ExPath, certain lesions and pathologies
previously diagnosed with an electron microscopy (EM) can now be diagnosed with a conventional optical microscope after
physical tissue expansion, an inexpensive, faster and reliable strategy. It also enables high-fidelity computational discrimination
between early neoplastic lesions that to date have challenged the human judgment.
Conclusion:
ExPath offers new approaches for assessing pathologically important features in human tissue. It may eliminate the
need for EM in the diagnosis of certain diseases for which EM is required for diagnosis and it can improve the computational
discrimination between pathological lesions that are hard to distinguish with existing techniques. ExPath may enable routine
use of nanoscale imaging in molecular pathology and research (2017, Nature Biotechnology).
Biography
Octavian Bucur, MD, PhD is Instructor in the Department of Pathology at the Harvard Medical School and Beth Israel Deaconess Medical Center, in Boston, MA,
focusing on the development and application of new experimental and computational technologies with significant impact in molecular, diagnostic pathology and
personalized medicine. He is also a member of the Ludwig Cancer Center at Harvard and Broad Institute of MIT and Harvard. In collaboration with Dr Edward
Boyden’s laboratory at MIT, he has developed a pathology-optimized physical tissue expansion method called Expansion Pathology, that enables ~100 times
expansion in volume of any type of clinical specimen and visualization of 70-80nm structures with conventional optical microscopes (currently limited to ~250nm
resolution). Expansion Pathology has the potential of replacing electron microscopy in diagnosis and investigation of certain pathologies and nanometer structures
(Nature Biotechnology, August 2017, 3 patents filed).
obucur@bidmc.harvard.eduOctavian Bucur
BIDMC, USA
Octavian Bucur, J Gastrointest Dig Syst 2018, Volume 8
DOI: 10.4172/2161-069X-C8-084