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conferenceseries
.com
Volume 9
Journal of Biotechnology & Biomaterials
ISSN: 2155-952X
JOINT EVENT
February 28-March 02, 2019 | Berlin, Germany
5
th
International Conference on
Enzymology and Protein Chemistry
&
22
nd
Global Congress on
Biotechnology
Biotechnology 2019
Enzymology 2019
February 28-March 02, 2019
&
Overexpression of a tomato annexin gene AnnSp2, enhances abiotic stress tolerance in transgenic
tomato through ABA synthesis and modulation of ROS production
Raina Ijaz
1
, Javeria Ejaz
1
, Muhammad Imtiaz
2
and
Taotao Wang
3
1
University of Poonch Rawalakot, Pakistan
2
Guangzhou University, China
3
Huazhong Agricultural University, China
D
rought and high salinity are two major abiotic stresses that affect the agricultural crops worldwide. Annexins belongs to a
multigene protein family that play an essential role in plant stress responses and various cellular processes. Here, AnnSp
2
gene was cloned from wild tomato (Solanum pennelli) and functionally characterized in cultivated tomato. AnnSp
2
was found
to be induced after exposure to drought, salt, H
2
O
2
and ABA. Tomato plants overexpressing AnnSp2 remarkably increased
plant tolerance to drought and salt stress, as determined through physiological analysis of the germination rate, root growth,
survival rate, leaf water loss and Chl content. AnnSp2 transgenic plants were observed to be less sensitive to ABA during seed
germination and seedling stages. However, under drought stress the ABA content significantly increased in the AnnSp
2
over
expressing plants, reduced water loss, attributed to the enhancement of stress tolerance. Furthermore, we found that AnnSl2
reduced sensitivity of plants to drought by influencing ABA induced stomatal movement and expression of ABA inducible
genes, including AREB, DREB, NCED, ERDwere clearly up regulated under drought and salt stress conditions. Consistent with
the accumulation of reactive oxygen species (ROS), lower lipid peroxidation level, increased peroxidase activities including
APX, CAT and SOD all of which contributed to increased tolerance to oxidative stress compared with wild-type plants. These
results therefore indicate that AnnSp
2
play an important role in the abiotic stress response and that overexpression of AnnSp2
in transgenic tomato improves salt and drought tolerance through ABA signalling and the regulation of ROS production in
plants.
Biography
Raina Ijaz is working as an Assistant Professor at University of Poonch Faculty of Agriculture, Rawalakot (Azad Kashmir), and focusing on the fate of biotechnology in
the horticultural plant vegetable science. She moved to Wuhan, China by the Scholarship Council (CSC) and finished her PhD under the guidance of Prof. Dr. Ye zhibiao
at Huazhong Agricultural University in 2017. She has published five research publications in various well reputed scientific impact factor journals. She has participated
in many national and international conferences, seminars and presented her research achievements. She is also serving as reviewer/referee for many national and
internationals journals.
rainaijaz@yahoo.comRaina Ijaz et al., J Biotechnol Biomater 2019, Volume 9
DOI: 10.4172/2155-952X-C2-115