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Volume 9
Journal of Biosensors & Bioelectronics
Biosensors Congress 2018
August 29-30, 2018
Page 12
conference
series
.com
August 29-30, 2018 Tokyo, Japan
9
th
World Congress on
Biosensors and Bioelectronics
Direct, label-free and rapid transistor-based immunodetection in whole serum
T
ransistor-based biosensors fulfill many requirements posed upon transducers for future point-of-care diagnostics devices
such as scalable fabrication, label-free and real-time quantification of chemical and biological species with high sensitivity.
However, the short Debye screening length in physiological samples (<1 nm) has been a major drawback so far, preventing
direct measurements in serum. In this work, we demonstrate how tailoring the sensing surface with short specific biological
receptors and a polymer Polyethylene Glycol (PEG) can strongly enhance the Debye length. The mechanism is explained in
terms of local desalting of the sample by the polymer layer. In addition, the sensor performance can be dramatically improved
if the measurements are performed at elevated temperatures (37
o
C instead of 21
o
C). With this novel approach, highly sensitive
and selective detection of a representative immunosensing parameter ‘human thyroid-stimulating hormone’ is shown over a
wide measuring range with sub-picomolar detection limits in whole serum. To our knowledge, this is the first demonstration
of direct immunodetection in whole serum using transistor-based biosensors without the need for sample pre-treatment and
labeling or washing steps. The presented sensor is low-cost, can be easily integrated into portable diagnostics devices and offers
a competitive performance compared to state-of-the-art central laboratory analyzers.
Biography
Oscar Gutierrez-Sanz has completed his PhD from Instituto de Catálisis y Petroleoquímica, CSIC. He is a Postdoctoral Researcher in Biomed X, an exciting new
collaboration model at the interface between academia and industry. He has published more than 17 papers in reputed journals and he is also a co-inventor/inventor
of three pending patents.
gutierrez@bio.mxOscar Gutierrez-Sanz
BioMed X Innovation Center, Germany
Oscar Gutierrez-Sanz, J Biosens Bioelectron 2018, Volume 9
DOI: 10.4172/2155-6210-C1-035