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Volume 7, Issue 2 (Suppl) J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal Biomaterials 2017
March 27-28, 2017
Page 27
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2nd Annual Conference and Expo on
March 27-28, 2017 Madrid, Spain
An improved biofabrication process to enhance cell survival and distribution in bioprinted scaffolds
for cartilage regeneration
Tissue regeneration (TR) is currently one of the most challenging biotechnology unsolved problems. Tissue engineering
(TE) is a multidisciplinary science that aims at solving the problems of TR. TE could solve pathologies and improve the
quality of life of billions of people around the world suffering from tissue damages. New advances in stem cell (SC) research
for the regeneration of tissue injuries have opened a new promising research field. However, research carried out nowadays
with two-dimensional (2D) cell cultures do not provide the expected results, as 2D cultures do not mimic the 3D structure
of a living tissue. Some of the commonly used polymers for cartilage regeneration are Poly-lactic acid (PLA) and its derivates
as Poly-L-lactic acid (PLLA), Poly(glycolic acids) (PGAs) and derivates as Poly(lactic-co-glycolic acids) (PLGAs) and Poly
caprolactone (PCL). All these materials can be printed using fused deposition modelling (FDM), a process in which a heated
nozzle melts a thermoplastic filament and deposits it in a surface, drawing the outline and the internal filling of every layer.
All these procedures uses melting temperatures that decrease viability and cell survival. Research groups around the world are
focusing their efforts in finding low temperature printing thermoplastics or restricted geometries that avoid the contact of the
thermoplastic and cells at a higher temperature than the physiologically viable. This mainly has 2 problems; new biomaterials
need a long procedure of clearance before they can be used in clinical applications, and restrictions in geometries will limit
the clinical application of 3D printing in TE. We have developed an enhanced printing process named Injection Volume
Filling (IVF) to increase the viability and survival of the cells when working with high temperature thermoplastics without
the limitation of the geometry. We have demonstrated the viability of the printing process using chondrocytes for cartilage
regeneration. This development will accelerate the clinical uptake of the technology and overcome the current limitation when
using thermoplastics as scaffolds.
Biography
José Manuel Baena, MSc is a ResearchAssociate. He is the Founder of BRECAHealth Care, pioneer in 3D printed custommade implants for orthopedic surgery, and RE-
GEMAT 3D, the first Spanish bioprinting company. He is an expert in innovation, business development and internationalization. He is a Lecturer in some business schools,
and is passionate about Biomedicine and Technology. In his free time, he also works as a Researcher at the Biopathology and Regenerative Medicine Institute (IBIMER).
José Manuel Baena
Universidad de Granada, Spain
José Manuel Baena, J Biotechnol Biomater 2017, 7:2 (Suppl)