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conferenceseries
.com
Volume 8, Issue 5 (Suppl)
J Bioremediat Biodegrad, an open access journal
ISSN: 2155-6199
Biofuels Congress 2017
September 05-06, 2017
September 05-06, 2017 | London, UK
Biofuels and Bioenergy
6
th
World Congress on
Towards the development of rapid biofilm antibiotic sensitivity testing (BAST)
Bassam O AlJohny
1
, AL Quthami Khalid
2
and
Milton Wainwright
3
1
King Abdulaziz University, Saudi Arabia
2
Regional Laboratory, Makkah, Saudi Arabia
3
University of Sheffield, UK
T
his study presents a modification of the antibiotic susceptibility testing (AST), which is a rapid means of determining the
response of planktonic bacteria to different antimicrobial agents, for application to biofilms. Colony biofilm was first developed
on a cellulose filter/membrane disc, over which an antibiotic disc was imposed. Zone of inhibition was measured after incubation
on nutrient agar. Biofilms were not as susceptible to test antibiotics as compared to the planktonic cultures. The results point to the
possibility of this method as a rapid means for antibiotics for treating biofilm infections. Limitations and potential application for
biofilm AST are discussed.
Biography
Bassam O AlJohny has completed his PhD from Sheffield University, England and has been trained on Molecular Techniques from Department of Molecular
Biology and Biotechnology, Sheffield University. Presently he has been working as Associate Professor in Molecular Microbiology, Department of Biology. He
is an energetic and enthusiastic Microbiologist and has 7 years’ experience in both teaching and research activity. He has participated in several international,
national conferences, symposia, workshops and presented his research papers in several countries and recently presented his novel research in Paris. He has
published more than 9 papers in reputed national, international and ISI journals and serving as an Editorial Board Member of reputed journals and also has lifetime
membership in Microbiology Associations worldwide.
boaljohny@kau.edu.saBassam O AlJohny et al., J Bioremediat Biodegrad 2017, 8:5(Suppl)
DOI: 10.4172/2155-6199-C1-008