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Volume 9
Journal of Bioremediation & Biodegradation
ISSN: 2155-6199
Biofuel Congress 2018 &
Biomass 2018
September 04-06, 2018
JOINT EVENT
September 04-06, 2018 | Zurich, Switzerland
13
th
Global Summit and Expo on
Biomass and Bioenergy
&
12
th
World Congress on
Biofuels and Bioenergy
Targeted Modulation of Brassica Seed Triglycerides Pathway to Produce Plant Oil for Direct Use as
Biodiesel
Iqbal Munir
1
, Ijaz Naeem
1
, Timothy P. Durrett
2
, Aqib Iqbal
1
, Mian Afaq Ahmad
1
, Raheel Munir
1
and
Fazli Zahir
1
1
The University of Agriculture Peshawar-Pakistan
2
Kansas State University, USA
E
nergy crises along with environmental concerns are driving researchers to develop viable alternative fuels from renewable
resources. The use of
Brassica juncea
oil as an alternative fuel suffers from problems such as high viscosity, low volatility
and poor cold temperature properties. The seed of
Euonymus alatus
produces low viscosity oil having unusual triacylglycerol
(TAGs) called acetyl triacylglycerol (acTAGs) where the sn-3 position is esterified with acetate instead of a long chain fatty
acid. The enzyme
Euonymous alatus
diacylglycerol acetyltransfrase (EaDacT) present in these plants is an acetyltransferase
that catalyzes the transfer of an acetyl group from acetyl-CoA to diacylglycerol (DAG) to produce acTAG. In order to reduce
the viscosity of
Brassica juncea
oil by synthesizing acTAG, we have developed an efficient and simple agrobacterium mediated
floral dip transformation method to generate transgenic
Brassica juncea
plants. A binary vector containing the EaDacT gene
under the transcriptional control of a glycinin promoter and with a basta selection marker was transformed into
Agrobacterium
tumefaciens
strain GV-3101 through electroporation. Basta is a herbicide which is used as a selection marker to allow us to
conveniently screen very young transgenic plants from a large number of untransformed plants. The basta resistant putative
transgenic plants were further confirmed by PCR. Biochemical analyses of the transgenic B. juncea seed revealed modified
fatty acids profile having no acetyl TAGs. Alternative strategy is in process to silence genes encoding enzymes DGAT/PDAT
along with overexpression of
EaDAcT,
that will hopefully produce acetyl TAGs.
iqmunir@aup.edu.pkJ Bioremediat Biodegrad 2018, Volume 9
DOI: 10.4172/2155-6199-C1-015