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conferenceseries.com
Volume 8 Journal of Ecosystem & Ecography Biodiversity Congress 2018
July 26-27, 2018
July 26-27, 2018 Melbourne, Australia
7th International Conference on
Biodiversity Conservation and Ecosystem Management
Metabarcoding and culturing to investigate the diverse fungi associated with cabbage in and their
bio-control potential
Michal Kuchár
Bio-Protection Research Centre, New Zealand
Statement of the Problem: Agricultural crops such as cabbage ( Brassica oleracea var. capitata) are vulnerable to a plethora of pests
and diseases. Endophytic fungi are increasingly used in biological control against these pests and diseases as agricultural producers
attempt to reduce their environmental footprint.
Methodology & Theoretical Orientation: In this study, putative endophytic fungi were isolated from cabbage grown in three
regions in New Zealand in order to identify isolates with potential to assist crop production. Selected fungal isolates were tested in
bioassays to determine their potential for biological control of a fungal disease caused by Sclerotinia sclerotiorum and an insect pest
of cabbage - Diamondback moth (Plutella xylostella). In addition to isolation from plants, patterns in fungal communities were
also described using metabarcoding data acquired by high-throughput sequencing and compared with data on direct isolation
through culturing.
Findings: Over 100 species were identified using culturing approaches, while metabarcoding approaches suggest the number of
fungal species associated with cabbage tissues could be over 200. Fungal communities differ in above and belowground parts of
cabbage. There does not seem to be a significant difference between fungal communities in different regions of New Zealand.
Increased mortality of Diamondback moth larvae feeding on cabbage leaves treated with spore suspensions of several isolates of
Lecanicillium sp. was observed. None of the tested isolates used as seed inoculants significantly reduced disease symptoms caused by
Sclerotinia sclerotiorum in a bioassay on cabbage seedlings.
Conclusion & Significance: In this study several isolates were identified as promising biological control agents of an insect pest of
cabbage. However, these isolates are members of a large consortium of fungi. The uncovered patterns in the fungal communities
underline the importance of understanding of the microbial communities associated with agricultural crop for us to improve it.
Biography
Michal Kuchár has completed his BSc in Biology and MSc in Plant Physiology from the Charles University in Prague in Czech Republic. His PhD at the Bio-
Protection Research Centre is aligned to the Next-Generation Biopesticides program and is focusing on fungal endophytes in Brassica species and their potential
use as bioprotection agents. He is isolating fungal endophytes from plants under different environmental and agricultural growth conditions and assessing the
factors that shape composition of fungal endophytic communities using next-generation sequencing techniques.
Michal Kuchár, J Ecosyst Ecogr 2018, Volume 8
DOI: 10.4172/2157-7625-C4-041