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24
th
Biotechnology Congress: Research & Innovations
CRISPR Cas9 Technology and Genetic Engineering
Annual Congress on
October 24-25, 2018 | Boston, USA
Journal of Biotechnology & Biomaterials | ISSN: 2155-952X | Volume: 8
Strategies for controlling off-target effects and biological variations in CRISPR/Cas9 genome editing
experiments
Michelle Kimberland
GlaxoSmithKline, USA
T
he CRISPR/Cas9 system has enabled efficient modification of genes in a variety of cellular systems for studying phenotypic
effects of genetic perturbations. However, various levels of off-target effects (OTEs) have been reported. It can be difficult
to conclusively determine that the observed phenotypic changes are in fact due to the intended modification of the target gene
and not from unintended mutations elsewhere in the genome. In addition, biological variations observed within cultured cells
can also confound results and need to be addressed. In this poster, designing and experimental strategies for minimizing and
controlling OTEs and biological variations in CRISPR genome editing experiments are summarized, together with orthogonal
approaches used to confirm on-target KO effects.
Biography
Michelle Kimberland has her background experience in molecular biology and genetics. She has worked on a variety of diseases/disease areas including hemophilia
A, hepatitis C viral replication and womens’ health issues. She is currently working at GlaxoSmithKline where her work is focused on genome editing for target
selection, target validation and phenotypic assay development.
michelle.l.kimberland@gsk.comMichelle Kimberland, J Biotechnol Biomater 2018, Volume 8
DOI: 10.4172/2155-952X-C4-098