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Journal of Biotechnology & Biomaterials | ISSN: 2155-952X | Volume: 8

Biotechnology Congress: Research & Innovations

CRISPR Cas9 Technology and Genetic Engineering

Annual Congress on

October 24-25, 2018 | Boston, USA

Dairy Effluent conversion into biofertilizer using tailor made microbial consortium

Statement of the Problem:

Dairy industry generates 3m

of effluent per m

of processed milk which comes mostly from the

cleaning process that uses fresh water. The effluent is nutrient rich and can cause an environmental problem unless properly

treated. The conventional treatment is tedious, energy intense, cost ineffective. Adopting this technology is a burden for the

larger establishments and crippling for the small-scale installations. Hence technologies are sought to make the process of

effluent treatment eco-friendly and economically viable.

Methodology & Theoretical Orientation:

Tailor-made consortium was developed for conversion of the nitrogenous waste

in dairy effluent into ammonia. The process was carried out using biofilm bioreactors in order to ensure one-time bacterial

charging with continued performance. The process was scaled to industrial scale (more than 5000 liters). The treated effluent

was used for field trial and pot trial for the cultivation of economic crops as per standard procedure.

Findings:

Tailor-made microbial consortium produced ammonia from dairy effluent at a rate of 1.66x10

-4

mol s

-1

within 1

hour of incubation in a biofilm bioreactor at 37

C with highest production at 16th hours of incubation (56.81mg/100ml)

demonstrating 95.7% ammonia production with 72.3% nitrate and a concomitant 33.2% phosphate reduction from an initial

load of 32-270ppm nitrate and 15-40ppm phosphate respectively with 82.55% BOD reduction in 16th hour, as compared with

66.6% in 48 hours through constructed wetlands. The treated effluent increased biomass in case of mung bean (Mb) and

Sorghum Sudan grass; decreased root nodulation while enhanced seed yield with improved protein and carbohydrate content

in Mb while providing protection from aphid infestation. This treated effluent significantly enhance the basal diameter and

fiber yield in case of Ramie, a plant of immense economic value. It could also enhance production in the case of potato, hence

functioning as a biofertilizer. This approach enables the conversion of effluent into a by-product of immense economic value

hence making the process of dairy effluent treatment self-sustainable. The process was scalable from 1liter to more than 5000

liters for treating actual dairy effluent with associated field trial.

Biography

Shaon Ray Chaudhuri from the Department of Microbiology has been working independently in the area of Microbial Technology since 2003. Her group has been

working on development waste water specific tailor made microbial consortium for treatment with minimum dead mass generation. Eight scholars have graduated;

two are in the process of being awarded their doctoral degree, while 6 scholars and 9 master’s students are working in the group to develop new solutions for waste

management with environmental sustenance. She has 5 technologies transferred, 4 awarded international patents while 10 filed patents.

shaonraychaudhuri@triprauniv.in

Shaon Ray Chaudhuri

Tripura University, India

Shaon Ray Chaudhuri, J Biotechnol Biomater 2018, Volume 8

DOI: 10.4172/2155-952X-C4-096