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conferenceseries
.com
Volume 8
Journal of Gastrointestinal & Digestive System
ISSN: 2161-069X
Bariatric Surgery 2018 & Gastro 2018
March 15-16, 2018
JOINT EVENT
12
th
Global Gastroenterologists Meeting
3
rd
International Conference on Metabolic and Bariatric Surgery
&
March 15-16, 2018 Barcelona, Spain
Effects of targeting SLC1A5 on inhibiting gastric cancer growth and tumor development
in vitro
and
in vivo
Chen Min, Sumeng Gao
and
Xiaoping Zou
The Affiliated Drum Tower Hospital - Nanjing University Medical School, P R China
Aims:
To investigate the oncogenic effects of SLC1A5 on gastric cancer development
in vitro
and
in vivo.
Methods:
The expression level of SLC1A5 was detected in 70 gastric cancer paraffin-embedded tissues by immunohistochemistry
and in gastric cancer cell lines by qRT-PCR and western blotting analysis. The effects of knockdown SLC1A5 were analyzed on cell
proliferation, cell cycle, the ability of cell migration and invasion and growth signaling pathways in vitro. By using subcutaneous
xenograft mouse, the importance of SLC1A5 expression was assessed for both successful engraftment and growth of gastric cancer
cells in vivo.
Results:
SLC1A5 is up-regulated in gastric cancer tissues and is correlated withmalignant features such as deeper local invasion, higher
lymph node metastasis, advanced TNM stages and higher Ki-67 expression. Knockdown SLC1A5 in gastric cancer cells suppressed
cell proliferation, caused G0/G1 arrest, inhibited cell invasion and migration partly by inactivated mTOR/p-70S6K1 signaling in vitro.
Furthermore, in vivo experiments indicated that suppression of SLC1A5 could inhibit relative volume of xenografted tumor.
Conclusions:
Our results suggest that SLC1A5 may represent a new biomarker and be a potential therapeutic target in gastric cancer.
Biography
Chen Min has completed her PhD from Wuhan University, School of Medicine. She is an associated Physician working in the affiliated Drum Tower Hospital of
Nanjing University, Medical School, P R China for almost seven years. She is In Charge of teaching work in the Department as Teaching Secretary. She has
published more than 10 papers in reputed journals. Her research field is about basic and clinical research in gastric cancer.
croweminchan@gmail.comChen Min et al., J Gastrointest Dig Syst 2018, Volume 8
DOI: 10.4172/2161-069X-C1-064