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Volume 9, Issue 11 (Suppl) J Cancer Sci Ther
ISSN: 1948-5956 JCST, an open access journal Asia Pacific Oncologists 2017
November 20-22, 2017
November 20-22, 2017 Melbourne, Australia
14th Asia Pacific
Oncologists Annual Meeting
SKA1: A therapeutic target for chemo-resistance in human osteosarcoma
Qiong Ma
Fourth Military Medical University, China
The combination of aggressive surgery and neoadjuvant chemotherapy is also the major treatment for human osteosarcoma
nowadays. However, the issue of chemo-resistance development has sustained and poses a great challenge. Researchers
have reported that hypoxia may lead to drug resistance in many kinds of solid tumors. The mechanism is not very clear yet. The
purpose of this study is to explore how hypoxia leading to chemo-resistance in osteosarcoma cells. We scanned normoxia and
hypoxia cultured osteosarcoma cells in silico in three replicates to find the differential expressed gene under hypoxic condition,
and this gene was overexpressed by lenti-virus vector, then real-time PCR and western were utilized to detect the expression of
three drug resistance related genes ABCB1, ABCC2, and GSTP1. Cell Counting Kit-8 (CCK8) assay was performed to evaluate
the proliferation of cells after lenti-virus transfected. 545 differentially expressed genes were identified based on the microarray
analysis. Attention was focused on the SKA1 gene as a possible downstream target of hypoxia by means of bioinformatics. SKA1
overexpression reduced the expression of three multidrug resistant genes ABCB1, ABCC2 and GSTP1. Also, we demonstrated
that SKA1 overexpression enhanced the sensitivity of two chemotherapeutic drugs used for osteosarcoma patients. Our study
made an attempt to identify the downstream target genes that are altered in the hypoxia-cultured osteosarcoma cell line by
microarray analysis. We demonstrated that the expression of SKA1 was significantly decreased in a hypoxic environment. And
SKA1 may function as a chemosensitizer in osteosarcoma. A strategy to enhance its expression may prove to be beneficial for
the treatment of osteosarcoma.
Biography
Qiong Ma has her expertise and interest in scientific study on human osteosarcoma, especially about the proliferation, invasion and metastases of tumor cells. She
has studied tumor cells under hypoxic conditions.
Qiong Ma, J Cancer Sci Ther 2017, 9:11 (Suppl)
DOI: 10.4172/1948-5956-C1-117