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Analytica 2016
September 28-30, 2016
Volume 7, Issue 5(Suppl) J Anal Bioanal Tech 2016
ISSN: 2155-9872 JABT, an open access journal
conferenceseries .com
September 28-30, 2016 Orlando, USA
7th International Conference and Exhibition on
Analytical & Bioanalytical Techniques
Satoru Michiyuki et al., J Anal Bioanal Tech 2016, 7:5(Suppl)
The development of semi-quantitative loop-mediated isothermal amplification (LAMP) assay using
multi-well chip
Satoru Michiyuki, Takaaki Ueda and Yasuyoshi Mori
Eiken Chemical Co., Japan
Nucleic acid amplification tests (NAATs) have become common tools for detecting pathogens in clinical samples. Among
NAATs, loop-mediated isothermal amplification (LAMP) assay, which enables DNA amplification and detection at
constant temperature, has the advantages of reaction simplicity, amplification efficiency and also inexpensiveness compared to
PCR-based technologies. Quantitative analysis in NAATs have been performed by kinetic analysis of amplification reactions
as in so-called “real-time PCR method” and this approach has found to be applicable to LAMP assay as well. However, these
assays require sophisticated instruments and well-trained laboratory staffs to obtain accurate and reproducible results. This
limitation has been a main obstacle to expand this type of quantitative LAMP assay to point of care tests in resource limiting
facilities. In this study, we developed simple and rapid semi-quantitative LAMP assay based on multi-well dispensing method
(multi-well qLAMP). In the presentation, we will demonstrate that our novel technology is sufficient to distinguish some
criterion of DNA levels with high reproducibility and highly correlative to conventional quantitative LAMP assay. We expect
that this technology can be applicable as point of care tests to help determination of treatment eligibility, especially in infectious
diseases whose amount of pathogenic DNA is a crucial criterion of defining treatment strategy.
Biography
Satoru Michiyuki has completed his Master’s degree from Kyushu University. He has worked as a Researcher focusing on the development of genetic clinical
diagnosis in Eiken Chemical Co., since 2011.