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.com
Volume 09
Journal of Addiction Research & Therapy
Addiction Summit 2018
May 17-18, 2018
May 17-18, 2018 Singapore
8
th
International Conference on
Addictive Disorders and Alcoholism
Effects of
Camellia sinensis
in alcohol abstinence anxiety, depression and gene expression analysis in
hippocampus of rats
Girdhari Lal Gupta
1
and Oseen Thakur
2
1
SVKM’s NMIMS University, India
2
Jaypee University of Information Technology, India
Statement of the Problem:
Alcoholism is a serious public health problem that often results in medical, social (violent crimes
and traffic accidents) and economic consequences throughout the world. There has been increasing thrust worldwide to opt
for safer and effective plant-derived anti-addiction drugs mentioned in the traditional medical systems. Green tea (
Camellia
sinensis
, family
Theaceae
) have huge medicinal uses and traditionally used for the treatment of alcohol disorders. Therefore,
we evaluated the effect of
Camellia sinensis
in alcohol abstinence syndrome, developed following long-term voluntary alcohol
intake in rats.
Methodology & Theoretical Orientation:
The hydro-alcoholic extract of
Camellia sinensis
leaves (HECS) was first
characterized for the presence of epigallocatechin gallate (15.73% w/w), and subsequently acute, sub-acute toxicity studies
were also performed. For evaluation of the effects of HECS in ethanol abstinence syndrome, healthy Wistar rats were enabled
to voluntary drinking of 9% v/v alcohol for 15 days. The behavior studies were conducted employing tail suspension test, forced
swim test, light-dark model and elevated-plus maze test on day 16
th
, 17
th
and 18
th
and peak ethanol withdrawal syndrome
were determined. HECS (50, 100 and 200 mg/kg) and standard drug diazepam were administered orally during withdrawal
symptoms. Oxidative stress parameters, serum serotonin, expression of
GABRA1, GABRA2, GABRA3, GABRA4, GABRA5
genes for GABA
A
receptor and
GRIN1, GRIN2A, GRIN2B
genes for NMDA-glutamate receptor in hippocampus of rats were
also determined.
Findings:
The results revealed that no observed adverse effect level was higher than 2000 mg/kg, orally. HECS exhibited
significant protective effect at doses 100 and 200mg/kg, but 50mg/kg showed insignificant protection against alcohol abstinence
syndrome like anxiety and depression. The increased expression of
GRIN2A, GRIN2B
and decreased expression of
GABRA1,
GABRA2, GABRA4
following ethanol abstinence were also reversed by HECS at doses 100 and 200 mg/kg.
Conclusion & Significance:
Thus, HECS has remarkable protective effects in ethanol abstinence syndrome, which may be due
to its antioxidant, serotonergic, GABAmimetic or anti-glutaminergic effect.
Biography
Girdhari Lal Gupta has received his MPharm degree in Pharmacology and PhD in Pharmaceutical Sciences in year 2005 and 2009, respectively. He is currently
working as an Associate Professor, Department of Pharmacology at SPP School of Pharmacy and Technology Management, SVKM’s NMIMS University, Mumbai,
India. He has published several scientific papers in peer-reviewed journals and presented his research work in conferences of national and international repute.
He has also received Young Scientists Award, Travel grants, best oral presentation award, Government funded research projects from Science Engineering and
Research Board, Indian Council of Medical Research. He is a Life Member of several professional bodies including Indian Pharmaceutical Association, Society for
Ethnopharmacology, Indian Pharmacological Society, Association of Pharmaceutical Teachers of India and Indian Pharmacy Graduates’ Association. He has also
been nominated as CPCSEA Member by Animal Welfare Division, Ministry of Environment, Forests and Climate Change, Government of India.
girdharilal.gupta@nmims.eduGirdhari Lal Gupta et al., J Addict Res Ther 2018, Volume 9
DOI: 10.4172/2155-6105-C1-036