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Volume 4, Issue 3 (Suppl) Adv Crop Sci Tech
ISSN: 2329-8863 ACST, an open access journal
Page 66
Notes:
Plant Genomics 2016
July 14-15, 2016
conferenceseries.com
July 14-15, 2016 Brisbane, Australia
4th International Conference on Plant Genomics
QTL identification for reproductive-stage salinity tolerance in rice using novel phenotyping technique
Rakesh K Singh1, Mostafa Ahmadizadeh 1, 2 and Naireen A Vispo1
1International Rice Research Institute, Philippines
2Sari Agricultural Sciences and Natural Resources University, Iran
Rice responds differentially at different stages of development. The seedling and reproductive stages are the most sensitive growth
stages with very weak association, suggesting that they are regulated by different processes and sets of genes/QTLs. There are
hardly any studies exist on reproductive-stage salinity tolerance mainly because of the lack of reliable reproductive-stage-specific
phenotyping techniques and incomplete knowledge of the stage-specific mechanisms of salinity tolerance. Two major challenges
for screening exclusively for the reproductive stage are; how to stress plants at the reproductive stage without stressing them at the
seedling or late vegetative stage; and how to impose the stress on different genotypes or mapping populations at equivalent growth
stages of development because of the variability in the progression of growth rate through developmental stages. We standardize
a methodology that allows salt translocation to the reproductive organs such as the flag leaf (the largest source for the sink) and
panicle as quickly as possible just at the initiation of booting when the genotypes are at the same stage of tissue/organ development
rather depending on age of the plant. A mapping population derived from CSR28/Sadri was used QTL identification using the
novel phenotyping technique and 6K illumina SNP platform. Salt stress equivalent to EC 10 dSm-1 was imposed on rice plants with
trimmed leaves starting from boot leaf emergence up to 20 days in an experiment. Stage-specific effect of salt stress was verified by
observing salt-sensitive and salt-tolerant genotypes. Details will be presented.
Biography
Rakesh K Singh is a Rice Breeder in Plant Breeding Division at IRRI, Philippines. His expertise is on breeding rice varieties for abiotic stress tolerance with special
reference to salt-affected soils (saline and alkaline soils), as well as zinc deficient and iron toxic soils. He has completed his MSc and PhD degree from GB Pant
University of Agriculture and Technology, Pantnagar, India and started his research career in 1986 as a Scientist at Central Soil Salinity Research Institute (Indian
Council of Agricultural Research), Karnal (India), where he strengthened the rice breeding program for salt-affected areas. He has moved to IRRI Philippines in
2005 and led the salinity breeding group to developed salinity tolerant and first time multiple stress tolerant (salinity+submergence tolerant) rice genotypes at IRRI
using molecular marker technology that are released and/or under advance stage of testing for release in various south and SE Asian countries. He has published
more than 75 papers in peer reviewed journals and guided many MS and PhD students from India, Philippines, Myanmar, Iran, Burundi, Madagascar and Egypt.
He is also serving as an Adjunct Associate Professor of Plant Breeding and Genetics at the University of the Philippines at Los Baños, Philippines.
Rakesh K Singh et al., Adv Crop Sci Tech 2016, 4:3 (Suppl)