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Volume 4, Issue 3 (Suppl) J Oral Hyg Health
ISSN: 2332-0702 JOHH, an open access journal
Page 45
Notes:
Dental Medicine 2016
August 08-10, 2016
conferenceseries.com
August 08-10, 2016 Toronto, Canada
13th International Conference and Exhibition on
Dental Medicine
Evaluation of in vitro biofilm removal with 2% and 10% sodium hypochlorite
Sayedah Zahra Rahmani, Dolatkhah H and Fattahi S
Behbood Hospital, Iran
Introduction: Biofilms are communities of microorganisms attached to a surface and embedded in a matrix of polysaccharides and
proteins forming a slimy layer. Oral bacteria have the capacity to form biofilms on distinct surfaces. Bacteria also form dense colonies
on root canal walls and features like isthmuses and lateral canals. Microbial communities in biofilms are remarkably difficult to
eradicate with antimicrobial agents for reasons that are yet to be adequately explained. Studies have shown that sodium hypochlorite
(NaOCl) is the most effective anti-microbial irrigant used during endodontic treatment. The aim of this study is to evaluate the
structure of biofilms and presence of EPS before and after the use of NaOCl 2% and 10%.
Materials & Methods: Dual species biofilms of Streptococcus oralis J22 and Actinomyces naeslundii T14VJ1 were grown under static
conditions and in a constant depth film fermentor (CDFF). Biofilms grown in the CDFF mimic the basal layer of an oral in vivo biofilm
better. For the statical conditions, a confined space was created over saliva coated dentin discs with supply of 20 ml of modified BHI
each 24 h for 4 and 10 days. For the CDFF, saliva coated hydroxyapatite discs biofilm was grown for 96 h at 37 uC under continuous
supply modified BHI at a rate of 45 ml/h. The system was equipped with 15 sample holders and each sample holder contained 5 saliva
coated hydroxyapatite discs, recessed to a depth of 250 mm. After growing the biofilms, NaOCl 2% and 10% were applied for 60 s and
300 s for removing the biofilm. Optical coherence tomography (OCT) was used for high-resolution, real-time imaging of a three-
dimensional structure of the biofilm. Confocal laser scanning microscopy (CLSM) was used to visualize the biofilm matrix, structure
and condition of bacteria (LIVE/DEAD staining).
Results: In the static biofilm group, OCT images showed reduction of biofilm thickness after applying the NaOCl 2% and 10% and
there was a very fluffy structure observable. In the CDFF group, OCT images showed bubble formation in the biofilm after using
NaOCl 10%, but the irrigant did not reduce the thickness of the biofilm or its consistency. The bubble formation was also observed in
CLSM images. The CLSM showed reduction of the biofilm structure but mostly living bacteria were found in the remaining biofilm.
Conclusion: According to our study, we need to use more efficient irrigants for more period of time to achieve our goal of cleansing
the matrix.
Biography
Sayedah Zahra Rahmani has earned scholarship for Dental School at Tabriz University of Medical Sciences and was graduated in 2015 and also she was the only
Dentistry student won the International Research Fellowship in Groningen, Netherlands in 2015. She has been working in Behbood Hospital as General Dentist in
2015 to till date. She is interested in research in medical and also basic science fields as well as dental practice.
Sayedah Zahra Rahmani et al., J Oral Hyg Health 2016, 4:3 (Suppl)