ISSN: 2278-0238
International Journal of Research and Development in Pharmacy & Life Sciences
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Agricultural Science
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Agricultural Science
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Agricultural Science
Agricultural science is a broad multidisciplinary field of biology that encompasses the parts of exact, natural, economic and social sciences that are used in the practice and understanding of agriculture.
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Agricultural Science
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Classically, the 3ââ¬â¢untranslated region (3ââ¬â¢UTR) is that region in eukaryotic protein-coding genes from the translation termination codon to the polyA signal. It is transcribed as an integral part of the mRNA encoded by the gene. However, there exists another kind of RNA, which consists of the 3ââ¬â¢UTR alone, without all other elements in mRNA such as 5ââ¬â¢UTR and coding region. The importance of independent 3ââ¬â¢UTR RNA (referred as I3ââ¬â¢UTR) was prompted by results of artificially introducing such RNA species into malignant mammalian cells. Since 1991, we found that the middle part of the 3ââ¬â¢UTR of the human nuclear factor for interleukin-6 (NF-IL6) or C/EBP gene exerted tumor suppression effect in vivo. Our subsequent studies showed that transfection of C/EBP 3ââ¬â¢UTR led to down-regulation of several genes favorable for malignancy and to up-regulation of some genes favorable for phenotypic reversion. Also, it was shown that the sequences near the termini of the C/EBP 3ââ¬â¢UTR were important for its tumor suppression activity. Then, the C/EBP 3ââ¬â¢UTR was found to directly inhibit the phosphorylation activity of protein kinase CPKC in SMMC-7721, a hepatocarcinoma cell line. Recently, an AU-rich region in the C/EBP 3ââ¬â¢UTR was found also to be responsible for its tumor suppression. Recently we have also found evidence that the independent C/EBP 3ââ¬â¢UTR RNA is actually exists in human tissues, such as fetal liver and heart, pregnant uterus, senescent fibroblasts etc. Through 1990ââ¬â¢s to 2000ââ¬â¢s, world scientists found several 3ââ¬â¢UTR RNAs that functioned as artificial independent RNAs in cancer cells and resulted in tumor suppression. Interestingly, majority of genes for these RNAs have promoter-like structures in their 3ââ¬â¢UTR regions, although the existence of their transcribed products as independent 3ââ¬â¢UTR RNAs is still to be confirmed. Our studies indicate that the independent 3ââ¬â¢UTR RNA is a novel non-coding RNA species whose function should be the regulation not of the expression of their original mRNA, but of some essential life activities of the cell as a whole.
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RELATIONSHIP BETWEEN OXIDATIVE STRESS AND ENERGY METABOLISM IN HYPERTENSION INDUCED CARDIAC REMODELING
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