ISSN: 2155-9872

Journal of Analytical & Bioanalytical Techniques
Open Access

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Study of Breath Acetone in a Rat Mode of 126 Rats with Type 1 Diabetes

Zhennan Wang1, Meixiu Sun1, Xiaomeng Zhao1, Chenyu Jiang1, Yingxin Li1 and Chuji Wang1,2*

1Institute of Biomedical Engineering, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China

2Department of Physics and Astronomy, Mississippi State University, MS 39759, USA

*Corresponding Author:
Chuji Wang
Department of Physics and Astronomy
Mississippi State University, MS 39759, USA
Tel: +16623259455
Fax: +16623258898
E-mail: cw175@msstate.edu

Received date: December 28, 2016; Accepted date: January 18, 2017; Published date: January 24, 2017

Citation: Wang Z, Sun M, Zhao X, Jiang C, Li Y, et al. (2017) Study of Breath Acetone in a Rat Mode of 126 Rats with Type 1 Diabetes. J Anal Bioanal Tech 8:344. doi: 10.4172/2155-9872.1000344

Copyright: © 2017 Wang Z, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

We investigate correlations of breath acetone with blood glucose (BG) and blood beta-hydroxybutyrate (BHB) in a rat model of 126 streptozotocin-induced Type 1 diabetic (T1D) and 32 healthy rats, and evaluate the role of breath acetone analysis as a non-invasive alternative in diabetes screening and management. We conducted breath acetone analysis using a T1D rat model under various conditions, including fasting and insulin-treated. Breath acetone concentrations were measured using a cavity ringdown breath acetone analyzer, LaserBreath-001. The results showed that the breath acetone, BG, and BHB in the T1D rat group were significantly different from those in the healthy group (P<0.05). Significant positive relationships between breath acetone and blood BHB existed in both T1D and healthy groups, as well as in a group of T1D rats under fasting condition and insulin treatment. When the BG concentrations were grouped as follows: 16.5-24.4, 24.5-27.7, and 27.8-41.7 mmol/L, a moderate negative correlation between breath acetone and BG was observed in the T1D group. Furthermore, the accuracy of diabetes screening using breath acetone was analyzed using the receiver operating characteristic (ROC) analysis method and results showed that the accuracy is low when the area under ROC curve (AUC) is <0.6425.

Keywords

Top