Optimization of EMS Mutagenesis on Petunia for TILLING
Received Date: Aug 27, 2014 / Accepted Date: Sep 23, 2014 / Published Date: Sep 23, 2014
Abstract
The development of a chemically-mutagenized population of Petunia hybrida could enable the identification of novel alleles for crop improvement. Conditions were determined for mutagenizing petunia with ethyl methanesulfonate (EMS), while minimizing deleterious effects on viability and fertility. A mutagenized population of the doubled haploid P. hybrida line ‘Mitchell Diploid’ was developed as a resource for TILLING. Three mutagenesis parameters were investigated in this study: the imbibition of seeds prior to EMS treatment, the EMS concentration, and EMS exposure time. Based on these results, the mutagenesis of 2000 petunia seeds for TILLING was conducted with a 12 hour imbibition followed by exposure to 0.1% EMS for 12 hours.
Keywords: Mutation breeding; Ethyl methanesulfonate; TILLING; Petunia
Citation: Jiang P, Chen Y, Wilde HD (2014) Optimization of EMS Mutagenesis on Petunia for Tilling, Ethiopia. Adv Crop Sci Tech 2:141. Doi: 10.4172/2329-8863.1000141
Copyright: © 2014 Jiang P, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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