ISSN: 2161-0681

Journal of Clinical & Experimental Pathology
Open Access

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
  • Research Article   
  • J Clin Exp Pathol 2017, Vol 7(6): 326
  • DOI: 10.4172/2161-0681.1000326

MUC1 and C-Met Affect Proliferation, Intercellular Junctions and Invasion in Two Head and Neck Carcinoma Cell Lines

Claudio Viveros-Amador1, Andrés Castell-Rodríguez2, Edgar Zenteno3, Juan Carlos Hernández-Guerrero1 and María Dolores Jiménez-Farfán1*
1Laboratorio de Inmunología, División de Estudios de Posgrado e Investigación, Facultad de Odontología, Universidad Nacional Autónoma de México. Circuito Investigación Científica s/n, , Ciudad Universitaria, Coyoacán, México City, CP. 04510, Mexico
2Laboratorio de Inmunoterapia e Ingeniería de Tejidos, Facultad de Medicina, Universidad Nacional Autónoma de México.Circuito Escolar s/n, , Ciudad Universitaria, Coyoacán, México City, CP. 04510, Mexico
3Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México.Circuito Escolar s/n, Ciudad Universitaria, , Coyoacán, México City, CP. 04510, Mexico
*Corresponding Author : María Dolores Jiménez-Farfán PhD., Laboratorio de Inmunología, División de Estudios de Posgrado e Investigación, Facultad de Odontología, Universidad Nacional Autónoma de México. Circuito Investigación Científica s/n, Ciudad Universitaria, Coyoacán, México City, CP. 04510, Mexico, Email: farfanmd@unam.mx

Received Date: Nov 10, 2017 / Accepted Date: Nov 23, 2017 / Published Date: Nov 27, 2017

Abstract

Role of metalloproteases and adhesion molecules has been studied in cancer and metastases; tyrosine kinase receptors (TKRs) and mucins are related to their expression.

Objective: To investigate the effects of MUC1/c-Met, and their participation in metastatic mechanism in head and neck carcinoma cell lines.

Materials and methods: Lines Cal27 and A253 from squamous cell carcinoma and submaxillary gland carcinoma were treated with SU11274 (c-Met inhibitor) and GO-201 (MUC1 inhibitor) and evaluated by western blot and immunocytochemistry with anti-claudin 1, 3, and 9, integrin-αVβ1, E-cadherin, MMP2, MMP9, TIMP1 and TIMP2 after inhibition. MMPs’ activity was assessed by zymography.

Results: Claudins were atypically located in the cytoplasm and nucleus and their expression is differentially modified. Migration and invasion rate were affected by inhibition. MMP9 activity was affected.

Conclusion: Our results suggest that the role of regulating MUC1 and c-Met is related to invasion mechanisms by dysregulation of claudins and MMPs activity.

Keywords: Head and neck carcinoma; Invasion; c-Met; MUC1; Claudins

Citation: Amador CV, Rodríguez AC, Zenteno E, Guerrero JCH, Farfán MDJ (2017) MUC1 and C-Met Affects Proliferation, Intercellular Junctions and Invasion in Two Head and Neck Carcinoma Cell Lines. J Clin Exp Pathol 7: 326. Doi: 10.4172/2161-0681.1000326

Copyright: ©2017 Amador CV, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Top