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Hindi Research Article
HPLC-DAD-ESI-MS/MS Identification and Characterization of Major Constituents of Iris crocea, Iris germanica and Iris spuria Growing in Kashmir Himalayas, India
Gulzar Bhat1, Abdul S Shawl1*, Zeeshan Shah1 and Mudasir Tantry21Natural Products Chemistry Division, CSIR-Indian Institute of Integrative Medicine, Srinagar, Kashmir, India
2Phytochemistry Laboratories, Centre of Research for Development, University of Kashmir, Srinagar, Kashmir, India
- *Corresponding Author:
- Abdul S Shawl
Natural Products Chemistry Division
CSIR-Indian Institute of Integrative Medicine Srinagar
Kashmir 190005, India
Tel: + 91 0194 2431509
Fax: + 91 0194 2441331
Email: asshawl@gmail.com
Received date: October 01, 2014; Accepted date: November 13, 2014; Published date: November 17, 2014
Citation: Bhat G, Shawl AS, Shah Z, Tantry M (2014) HPLC-DAD-ESI-MS/ MS Identification and Characterization of Major Constituents of Iris crocea, Iris germanica and Iris spuria Growing in Kashmir Himalayas, India. J Anal Bioanal Tech 5:223. doi: 10.4172/2155-9872.1000223
Copyright: © 2014 Bhat G, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Genus Iris has long history of use in various indigenous systems of medicine as alternative aperient, stimulant, cathartic, diuretic, gall bladder diseases, liver complaints, dropsy, purification of blood, venereal infections, fever, ringworms, bilious infections and for a variety of heart diseases. Rhizomes of genus Iris are rich source of secondary metabolites and most of these metabolites are reported to possess anticancer, antiplasmodial, anticholinesterase, enzyme inhibitor and immunomodulatory properties. In our study, high performance liquid chromatography with diode array detector coupled with electrospray ionization mass spectrometry (HPLC-DAD-ESI-MS/MS) method was established for simultaneous identification of flavonoids and other constituents in the rhizomes of three Iris species (Iris crocea, Iris germanica and Iris spuria) growing in Kashmir Himalayas, India. About twenty seven compounds were identified in these Iris species based on chromatographic retention time (tR), UV and MS/MS spectra and compared with those of isolated authentic compounds and literature data. About seven constituents (iridalglycoside 5b, iridalglycoside 7, irisquin B, irilin A, hesperetin, irisolone and irisolodone) were reported for the first time from Iris crocea. Our results demonstrate the developed method could be employed as a rapid and versatile analytical technique for identification of chemical constituents and quality control of Iris samples.
