Abstract

Over the years, there have been numerous attempts to develop new green synthesis technologies. Precious metals like copper, zinc, titanium, magnesium, silver, gold, and platinum are used to make nanoparticles. They have drawn a lot of interest due to their versatility as theragnostic agents. Significant antibacterial efficacy against Escherichia coli and Staphylococcus aureus, as well as antifungal activity against Trichosporon seinelii and Candida albicans, has been demonstrated by silver nanoparticles (Ag NP). AgNPs function in both drug delivery and successfully inducing the death of cancer cells. With the addition of Enterobacteriaceae cell filtrate (Escherichia coli, Klebsiella pneumonia, and Enterobacter cloacae) to AgNO3 solution, the silver ions are rapidly reduced within 5 minutes. The Temperature, pH, and AgNO3 concentration all influence the size of AgNPs generated using Escherichia coli, Klebsiella pneumoniae and Enterobacter cloacae, all of which create AgNPs. Since pH is essential for the effective synthesis of nanoparticles, this element increases the reactivity of plant extract with silver ions. Silver nanoparticle dilutions of 10,20,40,80,160 g/ ml were prepared and used in this analysis. 200μl of bacterial suspension was inoculated into each test tube containing varying amounts of AgNPs and a comparable volume of Muller Hinton Broth (MHB) and incubated for 24 hours at 37°C.

Keywords: Green Synthesis; Fourier-transform infrared spectroscopy; Transmission electron microscopy; Silver nanoparticle