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Hindi Research Article
Effects of Surface Roughness of Hydroxyapatite on Cell Attachment and Proliferation
Ling Li1, Kyle Crosby2, Monica Sawicki1, Leon L. Shaw1,2* and Yong Wang21Department of Mechanical, Materials and Aerospace Engineering, Illinois Institute of Technology, Chicago 60616, USA
2Department of Chemical, Materials, and Biomolecular Engineering, University of Connecticut, Storrs, CT 06269, USA
- Corresponding Author:
- Leon L. Shaw
Department of Mechanical
Materials and Aerospace Engineering
Illinois Institute of Technology, Chicago, IL 60616, USA
Tel: 312-567-3844
Fax: 312-567-7230
E-mail: lshaw2@iit.edu
Received date: August 24, 2012; Accepted date: September 25, 2012; Published date: September 28, 2012
Citation: Li L, Crosby K, Sawicki M, Shaw LL, Wang Y (2012) Effects of Surface Roughness of Hydroxyapatite on Cell Attachment and Proliferation. J Biotechnol Biomater 2:150. doi:10.4172/2155-952X.1000150
Copyright: © 2012 Li L, This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Hydroxyapatite (HA) is the main inorganic component of human hard tissue. It is bioactive and supports bone in growth and osteointegration, and thus it is widely used for orthopedic, dental and maxillofacial applications. To fully utilize its potential in forming a strong bone-implant interface, we have investigated the effect of surface roughness of HA on cell attachment and cell proliferation. The HA samples are prepared via sintering, followed by polishing and then scratch generation using a SiC metallographic paper. Cell attachment and proliferation properties are investigated with the aid of ROS 17/2.8 cells and Live/Dead cell staining, while cell morphologies on different surfaces are studied using scanning electron microscopy. It is found that rougher surfaces are better in enhancing cell attachment and proliferation than polished counterparts. It is proposed that the observed phenomenon is related to the enhanced availability of the medium and serum proteins through the grooves underneath the attached cells.
