ISSN: 2155-9872

Journal of Analytical & Bioanalytical Techniques
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  • Research Article   
  • J Anal Bioanal Tech 2017, Vol 8(6): 393
  • DOI: 10.4172/2155-9872.1000393

A New Heterobifunctional Cross-linker Based on an “Introverted” Acid: Mass Spectrometric and Bioinformatics Studies, Analysis of Intermolecular Crosslinking of Proteins

Sujeet Thakur K and Eswaran SV*
Regional Centre for Biotechnology, NCR Biotech Science Cluster, , Faridabad, Haryana, India
*Corresponding Author : Eswaran SV, Regional Centre for Biotechnology (Established by DBT, Government of India under the auspices of UNESCO), NCR Biotech Science Cluster, 3rd Milestone, Faridabad-Gurgaon Expressway, Faridabad, Haryana-121 001, India, Tel: +911292848888, Email: samba.eswaran@rcb.res.in

Received Date: Dec 27, 2017 / Accepted Date: Dec 29, 2017 / Published Date: Dec 30, 2017

Abstract

A new NHS-aryl azido heterobifunctional cross-linker based on an “introverted” carboxylic acid has been used to bring about successful intermolecular cross-linking. As a ‘proof-of-concept’ Lysozyme was incubated with the crosslinker, then photolysed (366 nm, 6 W UV lamp), subjected to SDS-PAGE, excision of the ‘dimer’, trypsin digested and analyzed by ESI-MS and StavroX 3.6.0.1. Previous studies on crosslinking of Lysozyme (SI-I and SIII) using homobifunctional cross-linkers, either no cross-linking was observed or only two crosslinks were detected in the case of BS3, a smaller cross-linker. The heterobifunctional cross-linker described here leads to many more crosslinks, which have been identified by using mass spectrometry (ESI-MS) and StavroX 3.6.0.1, a bioinformatics software, especially suited for identifying intermolecular crosslinking.

Keywords: Intermolecular crosslinking; SDS-PAGE; ESI-MS; StavroX 3.6.0.1

Citation: Thakur KS, Eswaran SV (2017) A New Heterobifunctional Cross-linker Based on an “Introverted” Acid: Mass Spectrometric and Bioinformatics Studies, Analysis of Intermolecular Crosslinking of Proteins. J Anal Bioanal Tech 8: 393. Doi: 10.4172/2155-9872.1000393

Copyright: ©2017 Thakur KS, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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