Abstract

One of the mitochondrial genes, cytochrome c oxidase I (COI), is a highly effective marker for DNA barcoding of many organisms. Using the Gene Elute DNA miniprep Kit, the COI was isolated from twelve distinct species of waterbirds. By creating a phylogenetic tree using the programme MEGA-X, the relationship between the waterbird species was evaluated. The phylogenetic tree’s dendrogram revealed two main branches, with the remaining five species aligning with two subgroups based on their identical COI sequences, and the seven species of water birds aligning one group with four subgroups.