Abstract

The biodegradation of dissolved organic acids (DOAs) in oil sands process-affected water (OSPW) presents a critical environmental challenge associated with bitumen extraction and processing operations. This study conducted a comprehensive laboratory analysis to investigate the in situ decomposition of DOAs in OSPW, aiming to elucidate the biogeochemical processes and microbial communities involved in organic matter degradation. Laboratory experiments simulated aerobic and anaerobic conditions representative of natural aquatic environments, with OSPW samples collected from extraction sites serving as the experimental substrate. Monitoring microbial growth and organic acid concentrations over time, coupled with molecular biology techniques, revealed significant biodegradation potential under both aerobic and anaerobic conditions.